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ab46666 |
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ab12188 has been referenced in 6 publications.
Publishing research using ab12188? Please let us know so that we can cite the reference in this datasheet
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ab12188 at 2ug/ml staining human thymus tissue sections by IHC-P. The tissue was formaldehyde fixed and then a heat mediated antigen retrieval step was carried out. The antibody was incubated with the tissue for 30 minutes. An HRP conjugated goat antibody was used as the secondary.
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Chromatin was prepared from mouse skin epidermis whole tissue lysate. Cells were fixed with 1% fromaldehyde for 10 minutes. TPA treated samples were incubated with rabbit IgG, HDAC7 antibody or ab12188 (diluted 1/800) for 12 hours at 4°C and the immunoprecipitated DNA was amplified using primers flanking the proximal promoter region and quantified by semiquantitative PCR. Rabbit IgG and another unrelated antibody immunoprecipitated sample did not produce significant amplification.
This image is courtesy of an anonymous Abreview
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