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Products:Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other
Overview
Product name
Anti-KAT2B / PCAF antibody - ChIP Grade
See all KAT2B / PCAF products (3) ...
Description
Rabbit polyclonal to KAT2B / PCAF - ChIP Grade
Tested applications
IHC-P, ChIP, WB, IP, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Dog, Human
Predicted to work with
Chicken
Immunogen
Synthetic peptide: EKFFFSKIKEAGLIDK (with N-terminal added cysteine) conjugated to KLH, corresponding to the C-terminal amino acids 817-832 of Human PCAF.
EKFFFSKIKE AGLIDK
Positive control
Immunoblotting: Whole extracts of human epidermoid carcinoma A431, dog MDCK kidney cells or recombinant active human PCAF. Immunoprecipitation: RIPA lysate, human acute T cell leukemia Jurkat cells.
General notes
If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Working dilutions should be discarded if not used within 12 hours.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: 1% BSA, 0.01M PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody is affinity-purified using the immunizing peptide immobilized on agarose.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HAT
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Microbiology >> Interspecies Interaction >> Host Virus Interaction
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab12188 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at an assay dependent dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ChIP: Use at an assay dependent dilution. (PubMed: 16829531Use at an assay dependent dilution.)
WB: 1/500Predicted molecular weight: 93 kDa.(1/500 (determined using whole extracts of human epidermoid carcinoma A431, dog MDCK kidney cells or recombinant active human PCAF and a chemiluminescent detection reagent). Predicted molecular weight: 93 kDa. In some extract preparations, additional band(s) may appear. Staining of PCAF band(s) by immunoblotting is inhibited by the PCAF immunizing peptide.)
IP: Use 5µg for 107 cells. (5-10µg of antibody immunoprecipitates PCAF from RIPA lysate with ~4 X 107 human acute T cell leukemia Jurkat cells.)
ICC/IF: Use at an assay dependent dilution. (PubMed: 20410122)
Target
Function
Functions as a histone acetyltransferase (HAT) to promote transcriptional activation. Has significant histone acetyltransferase activity with core histones (H3 and H4), and also with nucleosome core particles. Inhibits cell-cycle progression and counteracts the mitogenic activity of the adenoviral oncoprotein E1A. In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes.
Tissue specificity
Ubiquitously expressed but most abundant in heart and skeletal muscle.
Sequence similarities
Belongs to the GCN5 family.
Contains 1 bromo domain.
Contains 1 N-acetyltransferase domain.
Domain
The bromodomain mediates binding to HIV-1 Tat.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ92831
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CAF antibody
- CREBBP associated factor antibody
- GCN5 antibody
see all
Database links
- Entrez Gene: 428441 Chicken
- Entrez Gene: 8850 Human
- Entrez Gene: 18519 Mouse
- Omim: 602303 Human
- SwissProt: Q92831 Human
- SwissProt: Q9JHD1 Mouse
- Unigene: 533055 Human
- Unigene: 255025 Mouse
see all
Anti-KAT2B / PCAF antibody - ChIP Grade images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PCAF antibody - ChIP Grade (ab12188)
ab12188 at 2ug/ml staining human thymus tissue sections by IHC-P. The tissue was formaldehyde fixed and then a heat mediated antigen retrieval step was carried out. The antibody was incubated with the tissue for 30 minutes. An HRP conjugated goat antibody was used as the secondary.
This image is courtesy of an anonymous Abreview
ChIP - KAT2B / PCAF antibody - ChIP Grade (ab12188)
Chromatin was prepared from mouse skin epidermis whole tissue lysate. Cells were fixed with 1% fromaldehyde for 10 minutes. TPA treated samples were incubated with rabbit IgG, HDAC7 antibody or ab12188 (diluted 1/800) for 12 hours at 4°C and the immunoprecipitated DNA was amplified using primers flanking the proximal promoter region and quantified by semiquantitative PCR. Rabbit IgG and another unrelated antibody immunoprecipitated sample did not produce significant amplification.
This image is courtesy of an anonymous Abreview
References for Anti-KAT2B / PCAF antibody - ChIP Grade (ab12188)
This product has been referenced in:
- Ogiwara Het al. Histone acetylation by CBP and p300 at double-strand break sites facilitates SWI/SNF chromatin remodeling and the recruitment of non-homologous end joining factors. Oncogene 30:2135-46 (2011). Human.Read more (PubMed: 21217779) »
- Katsuno Met al. Disrupted transforming growth factor-beta signaling in spinal and bulbar muscular atrophy. J Neurosci 30:5702-12 (2010). WB, ICC/IF, IP; Human.Read more (PubMed: 20410122) »
See all 6 publications for this product
Publishing research using ab12188? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"