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Anti-KAT2B / PCAF antibody - ChIP Grade (ab12188)

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Overview

Product name

Anti-KAT2B / PCAF antibody - ChIP Grade
See all KAT2B / PCAF products (3) ...

Description

Rabbit polyclonal to KAT2B / PCAF - ChIP Grade

Tested applications

IHC-P, ChIP, WB, IP, ICC/IFmore details

Cross reactivity

Reacts with

Mouse, Dog, Human

Predicted to work with

Chicken

Immunogen

Synthetic peptide: EKFFFSKIKEAGLIDK (with N-terminal added cysteine) conjugated to KLH, corresponding to the C-terminal amino acids 817-832 of Human PCAF.

EKFFFSKIKE AGLIDK

Positive control

Immunoblotting: Whole extracts of human epidermoid carcinoma A431, dog MDCK kidney cells or recombinant active human PCAF. Immunoprecipitation: RIPA lysate, human acute T cell leukemia Jurkat cells.

General notes

If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 15mM Sodium Azide
Constituents: 1% BSA, 0.01M PBS, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

The antibody is affinity-purified using the immunizing peptide immobilized on agarose.

Clonality

Polyclonal

Isotype

IgG

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PCAF antibody - ChIP Grade (ab12188)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PCAF antibody - ChIP Grade (ab12188) image (enlarge)

  • ChIP - KAT2B / PCAF antibody - ChIP Grade (ab12188)ChIP - KAT2B / PCAF antibody - ChIP Grade (ab12188) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab12188 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • IHC-P: Use at an assay dependent dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • ChIP: Use at an assay dependent dilution. (PubMed: 16829531Use at an assay dependent dilution.)

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    WB

     WB: 1/500Predicted molec...Read more →

    WB: 1/500Predicted molecular weight: 93 kDa.(1/500 (determined using whole extracts of human epidermoid carcinoma A431, dog MDCK kidney cells or recombinant active human PCAF and a chemiluminescent detection reagent). Predicted molecular weight: 93 kDa. In some extract preparations, additional band(s) may appear. Staining of PCAF band(s) by immunoblotting is inhibited by the PCAF immunizing peptide.)

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    IP

      See more...Read more →

    IP: Use 5µg for 107 cells. (5-10µg of antibody immunoprecipitates PCAF from RIPA lysate with ~4 X 107 human acute T cell leukemia Jurkat cells.)

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    ICC/IF

     ICC/IF: Use at an assay ...Read more →

    ICC/IF: Use at an assay dependent dilution. (PubMed: 20410122)

Target

Function

Functions as a histone acetyltransferase (HAT) to promote transcriptional activation. Has significant histone acetyltransferase activity with core histones (H3 and H4), and also with nucleosome core particles. Inhibits cell-cycle progression and counteracts the mitogenic activity of the adenoviral oncoprotein E1A. In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes.

Tissue specificity

Ubiquitously expressed but most abundant in heart and skeletal muscle.

Sequence similarities

Belongs to the GCN5 family.
Contains 1 bromo domain.
Contains 1 N-acetyltransferase domain.

Domain

The bromodomain mediates binding to HIV-1 Tat.

Cellular localization

Nucleus.

Target information above from: UniProt accessionQ92831 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • CAF antibody
  • CREBBP associated factor antibody
  • GCN5 antibody
  • GCN5L antibody
  • GCN5L1 antibody
  • Histone acetylase PCAF antibody
  • Histone acetyltransferase KAT2B antibody
  • Histone acetyltransferase PCAF antibody
  • K(lysine) acetyltransferase 2B antibody
  • KAT2B antibody
  • KAT2B_HUMAN antibody
  • Lysine acetyltransferase 2B antibody
  • P antibody
  • P/CAF antibody
  • p300/CBP associated factor antibody
  • P300/CBP-associated factor antibody
  • Pcaf antibody
see all

Anti-KAT2B / PCAF antibody - ChIP Grade images:

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PCAF antibody - ChIP Grade (ab12188)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PCAF antibody - ChIP Grade (ab12188)

ab12188 at 2ug/ml staining human thymus tissue sections by IHC-P. The tissue was formaldehyde fixed and then a heat mediated antigen retrieval step was carried out. The antibody was incubated with the tissue for 30 minutes. An HRP conjugated goat antibody was used as the secondary.

This image is courtesy of an anonymous Abreview

See Abreview

  ChIP - KAT2B / PCAF antibody - ChIP Grade (ab12188)

ChIP - KAT2B / PCAF antibody - ChIP Grade (ab12188)

Chromatin was prepared from mouse skin epidermis whole tissue lysate.  Cells were fixed with 1% fromaldehyde for 10 minutes.  TPA treated samples were incubated with rabbit IgG, HDAC7 antibody or ab12188 (diluted 1/800) for 12 hours at 4°C and the immunoprecipitated DNA was amplified using primers flanking the proximal promoter region and quantified by semiquantitative PCR.  Rabbit IgG and another unrelated antibody immunoprecipitated sample did not produce significant amplification.

This image is courtesy of an anonymous Abreview

See Abreview

References for Anti-KAT2B / PCAF antibody - ChIP Grade (ab12188)

This product has been referenced in:

  • Ogiwara Het al. Histone acetylation by CBP and p300 at double-strand break sites facilitates SWI/SNF chromatin remodeling and the recruitment of non-homologous end joining factors. Oncogene 30:2135-46 (2011). Human.Read more (PubMed: 21217779) »
  • Katsuno Met al. Disrupted transforming growth factor-beta signaling in spinal and bulbar muscular atrophy. J Neurosci 30:5702-12 (2010). WB, ICC/IF, IP; Human.Read more (PubMed: 20410122) »

See all 6 publications for this product

Publishing research using ab12188? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"