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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PCAF antibody - ChIP Grade (ab12188)
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ChIP - KAT2B / PCAF antibody - ChIP Grade (ab12188)
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Product Name
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KAT2B / PCAF antibody - ChIP Grade
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See all KAT2B / PCAF antibodies (2)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to KAT2B / PCAF - ChIP Grade
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Immunogen
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Synthetic peptide: EKFFFSKIKEAGLIDK (with N-terminal added cysteine) conjugated to KLH, corresponding to the C-terminal amino acids 817-832 of Human PCAF.
BLAST 'EKFFFSKIKEAGLIDK' with  or 
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Reacts with
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Hu, Ms, Dog
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Predicted to react
(species key)
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Chk
(Due to sequence homology)
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Tested applications
(see key)
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ChIP, IHC-P, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ChIP: Use at an assay dependent dilution. See Sammons et al., 2006
IHC-P: Use neat. Perform heat mediated antigen retrieval before commencing with IHC staining protocol (see details in Abreview).
IP: 5-10µg of antibody immunoprecipitates PCAF from RIPA lysate with ~4 X 107 human acute T cell leukemia Jurkat cells.
WB: 1/500 (determined using whole extracts of human epidermoid carcinoma A431, dog MDCK kidney cells or recombinant active human PCAF and a chemiluminescent detection reagent). Predicted molecular weight: 93 kDa. In some extract preparations, additional band(s) may appear. Staining of PCAF band(s) by immunoblotting is inhibited by the PCAF immunizing peptide.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Immunoblotting: Whole extracts of human epidermoid carcinoma A431, dog MDCK kidney cells or recombinant active human PCAF.
Immunoprecipitation: RIPA lysate, human acute T cell leukemia Jurkat cells.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HAT
Chromatin and Nuclear Signaling >> Transcription >> Other factors
Microbiology >> Interspecies Interaction >> Host Virus Interaction
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other
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Relevance
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KAT2B / PCAF functions as a histone acetyltransferase to promote transcriptional activation. KAT2B specifically transfers acetyl onto histone H3 at K9, K14 and K18.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 15mM Sodium Azide
Constituents: 1% BSA, 0.01M PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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The antibody is affinity-purified using the immunizing peptide immobilized on agarose.
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Form
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Liquid
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Concentration
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1.300 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Notes
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If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Working dilutions should be discarded if not used within 12 hours.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this KAT2B / PCAF antibody - ChIP Grade is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for KAT2B / PCAF antibody - ChIP Grade images, references, products related to ab12188 and other tools.
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KAT2B / PCAF antibody - ChIP Grade images:
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 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PCAF antibody - ChIP Grade (ab12188)
ab12188 at 2ug/ml staining human thymus tissue sections by IHC-P. The tissue was formaldehyde fixed and then a heat mediated antigen retrieval step was carried out. The antibody was incubated with the tissue for 30 minutes. An HRP conjugated goat antibody was used as the secondary. This image is courtesy of an anonymous Abreview
See Abreview
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ChIP - KAT2B / PCAF antibody - ChIP Grade (ab12188)
Chromatin was prepared from mouse skin epidermis whole tissue lysate. Cells were fixed with 1% fromaldehyde for 10 minutes. TPA treated samples were incubated with rabbit IgG, HDAC7 antibody or ab12188 (diluted 1/800) for 12 hours at 4°C and the immunoprecipitated DNA was amplified using primers flanking the proximal promoter region and quantified by semiquantitative PCR. Rabbit IgG and another unrelated antibody immunoprecipitated sample did not produce significant amplification. This image is courtesy of an anonymous Abreview
See Abreview
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References for KAT2B / PCAF antibody - ChIP Grade (ab12188)
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This product has been used in: (two most recent references)
Drobic B et al. Promoter chromatin remodeling of immediate-early genes is mediated through H3 phosphorylation at either serine 28 or 10 by the MSK1 multi-protein complex. Nucleic Acids Res : (2010). ChIP; Human, Mouse. PubMed: 20129940
Rybtsova N et al. Transcription-coupled deposition of histone modifications during MHC class II gene activation. Nucleic Acids Res 35:3431-41 (2007). PubMed: 17478518
See all 5 publication references for this product.
If you publish research using ab12188 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to KAT2B / PCAF
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KAT2B / PCAF antibody - ChIP Grade - more information
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