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Products:Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other
Overview
Product name
Anti-KAT2B / PCAF antibody
See all KAT2B / PCAF products (3) ...
Description
Mouse monoclonal to KAT2B / PCAF
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Recombinant fragment: LEEEVYSQNS PIWDQDFLSA SSRTSQLGIQ TVINPPPVAG TISYNSTSSS LEQPNAGSSS PACKASSGLE ANPGEKRKMT DSHVLEEAKK PRVMGDIPME , corresponding to amino acids 353-452 of Human PCAF
LEEEVYSQNS PIWDQDFLSA SSRTSQLGIQ TVINPPPVAG TISYNSTSSS LEQPNAGSSS PACKASSGLE ANPGEKRKMT DSHVLEEAKK PRVMGDIPME
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Isotype
IgG2b
Light chain type
kappa
Research areas
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HAT
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Microbiology >> Interspecies Interaction >> Host Virus Interaction
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other
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Western blot - PCAF antibody (ab56535)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab56535 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: Use at a concentration of 1-5 µg/ml.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Functions as a histone acetyltransferase (HAT) to promote transcriptional activation. Has significant histone acetyltransferase activity with core histones (H3 and H4), and also with nucleosome core particles. Inhibits cell-cycle progression and counteracts the mitogenic activity of the adenoviral oncoprotein E1A. In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes.
Tissue specificity
Ubiquitously expressed but most abundant in heart and skeletal muscle.
Sequence similarities
Belongs to the GCN5 family.
Contains 1 bromo domain.
Contains 1 N-acetyltransferase domain.
Domain
The bromodomain mediates binding to HIV-1 Tat.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ92831
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CAF antibody
- CREBBP associated factor antibody
- GCN5 antibody
see all
Database links
- Entrez Gene: 8850 Human
- Omim: 602303 Human
- SwissProt: Q92831 Human
- Unigene: 533055 Human
Anti-KAT2B / PCAF antibody images:
Western blot - PCAF antibody (ab56535)
Western blot against tagged recombinant protein immunogen using ab56535 PCAF antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa
References for Anti-KAT2B / PCAF antibody (ab56535)
ab56535 has not yet been referenced specifically in any publications.
Publishing research using ab56535? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"