Anti-KAT2B / PCAF antibody (ab96510)
Key features and details
- Rabbit polyclonal to KAT2B / PCAF
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-KAT2B / PCAF antibody -
Description
Rabbit polyclonal to KAT2B / PCAF -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- 293T, A431, H1299, HeLa S3, HepG2, MOLT4, Raji cells, HeLa cell lysates
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab96510 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 93 kDa.
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ICC/IF |
1/100 - 1/200.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 93 kDa. |
ICC/IF
1/100 - 1/200. |
Target
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Function
Functions as a histone acetyltransferase (HAT) to promote transcriptional activation. Has significant histone acetyltransferase activity with core histones (H3 and H4), and also with nucleosome core particles. Inhibits cell-cycle progression and counteracts the mitogenic activity of the adenoviral oncoprotein E1A. In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes. -
Tissue specificity
Ubiquitously expressed but most abundant in heart and skeletal muscle. -
Sequence similarities
Belongs to the GCN5 family.
Contains 1 bromo domain.
Contains 1 N-acetyltransferase domain. -
Domain
The bromodomain mediates binding to HIV-1 Tat. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 8850 Human
- Entrez Gene: 18519 Mouse
- Omim: 602303 Human
- SwissProt: Q92831 Human
- SwissProt: Q9JHD1 Mouse
- Unigene: 533055 Human
- Unigene: 255025 Mouse
- Unigene: 483572 Mouse
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Alternative names
- CAF antibody
- CREBBP associated factor antibody
- GCN5 antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab96510 has been referenced in 2 publications.
- Shirakawa K et al. Salicylate, diflunisal and their metabolites inhibit CBP/p300 and exhibit anticancer activity. Elife 5:N/A (2016). WB . PubMed: 27244239
- Rabhi N et al. KAT2B Is Required for Pancreatic Beta Cell Adaptation to Metabolic Stress by Controlling the Unfolded Protein Response. Cell Rep 15:1051-1061 (2016). PubMed: 27117420