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Products:Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other
Overview
Product name
KAT2B / PCAF protein (Human)
See all KAT2B / PCAF products (4) ...
Protein description
Recombinant full length human KAT2B / PCAF expressed in baculovirus system; amino acids 1-832, 93kDa. Swiss ID = Q92831
Properties
Purity
Immunogen affinity purified
Purification notes
ab82074 is purified by an affinity column in combination with FPLC chromatography and is greater than 95% homogeneous. ab82074 contains no detectable proteases, DNase and RNase activity.
Biological activity
1 unit equals 1 nanogram of purified protein.
Form
Liquid
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 20% Glycerol, 20mM Tris HCl, 100mM Potassium chloride, 1mM DTT, 0.2mM EDTA, pH 8
Concentration
Concentration information loading...
Research areas
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HAT
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Microbiology >> Interspecies Interaction >> Host Virus Interaction
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab82074 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Gel supershift assays
Application notes
GSA: Use 1-5 units in a 20µl reaction.
Protein-protein interaction assay: Use 100-200 units.
in vitro transcription assay: Use 50-100 units.
in vitro acetylation assay: Use 100-200 units.
Cell growth assay: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Protein info
Function
Functions as a histone acetyltransferase (HAT) to promote transcriptional activation. Has significant histone acetyltransferase activity with core histones (H3 and H4), and also with nucleosome core particles. Inhibits cell-cycle progression and counteracts the mitogenic activity of the adenoviral oncoprotein E1A. In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes.
Tissue specificity
Ubiquitously expressed but most abundant in heart and skeletal muscle.
Sequence similarities
Belongs to the GCN5 family.
Contains 1 bromo domain.
Contains 1 N-acetyltransferase domain.
Domain
The bromodomain mediates binding to HIV-1 Tat.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ92831
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
References for KAT2B / PCAF protein (Human) (ab82074)
ab82074 has not yet been referenced specifically in any publications.
Publishing research using ab82074? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"