Anti-KAT3A / CBP antibody - ChIP Grade (ab10490)

Overview

• Product nameAnti-KAT3A / CBP antibody - ChIP GradeSee all KAT3A / CBP primary antibodies ...
• Description
  Rabbit polyclonal to KAT3A / CBP - ChIP Grade
• Tested applicationsIP, ChIP more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse
• Immunogen

  Immunogen was a synthetic peptide, which represented a portion of human CREB binding protein encoded within exon 33 (LocusLink ID 1387)(between residues 2392 and the c-terminus (residue 2442) according to Swiss-Prot entry Q92793).

• General notes

  
  

  Cyclic AMP-responsive enhancer binding protein (CREB) binding protein (CBP) and p300 are closely related transcriptional coactivators that have been shown to directly interact with many different DNA-binding transcription factors including nuclear hormone receptors, CREB (cyclic AMP-responsive enhancer binding protein), c-Fos, c-Jun/v-Jun, c-Myb/v-Myb, TFIIB and MyoD.Both CBP and p300 have been shown to display histone acetyltransferase (HAT) activity, capable of acetylating all four core histone particles in nucleosomes.As a result of HAT activity, it has been suggested CBP and p300 may play a direct role in activating chromatin for transcription.Single point mutations in CBP have been proposed as causative factors in the developmental abnormalities of Rubinstein-Taybi syndrome (RTS).Although both CBP and p300 appear to function similarly, the inability of p300 to rescue CBP malfunction iRTS suggests intrinsic functional differences between CBP and p300.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.1% Sodium Azide
  Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesAntibodies were affinity purified using the peptide immobilized on solid support.
• Primary antibody notes Cyclic AMP-responsive enhancer binding protein (CREB) binding protein (CBP) and p300 are closely related transcriptional coactivators that have been shown to directly interact with many different DNA-binding transcription factors including nuclear hormone receptors, CREB (cyclic AMP-responsive enhancer binding protein), c-Fos, c-Jun/v-Jun, c-Myb/v-Myb, TFIIB and MyoD.Both CBP and p300 have been shown to display histone acetyltransferase (HAT) activity, capable of acetylating all four core histone particles in nucleosomes.As a result of HAT activity, it has been suggested CBP and p300 may play a direct role in activating chromatin for transcription.Single point mutations in CBP have been proposed as causative factors in the developmental abnormalities of Rubinstein-Taybi syndrome (RTS).Although both CBP and p300 appear to function similarly, the inability of p300 to rescue CBP malfunction iRTS suggests intrinsic functional differences between CBP and p300.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Lipid and lipoprotein metabolism
  • Lipid metabolism

  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Pathways and Processes
  • Metabolism processes
  • Hypoxia

  • Stem Cells
  • Signaling Pathways
  • Wnt
  • Nuclear

  • Stem Cells
  • Signaling Pathways
  • Notch
  • Nuclear

  • Cancer
  • Cancer Metabolism
  • Response to hypoxia

  • Cancer
  • Cancer Metabolism
  • Metabolic signaling pathway
  • Metabolism of lipids and lipoproteins

  • Microbiology
  • Interspecies Interaction
  • Host Virus Interaction

  • Epigenetics and Nuclear Signaling
  • ChIP'ing antibodies
  • ChIP'ing antibodies

  • Epigenetics and Nuclear Signaling
  • Nuclear Signaling Pathways
  • Nuclear Receptors
  • Co-activators/co-repressors

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Other factors

  • Epigenetics and Nuclear Signaling
  • Chromatin Modifying Enzymes
  • Acetylation
  • HAT

Applications

Target

• FunctionAcetylates histones, giving a specific tag for transcriptional activation. Also acetylates non-histone proteins, like NCOA3 coactivator. Binds specifically to phosphorylated CREB and enhances its transcriptional activity toward cAMP-responsive genes. Acts as a coactivator of ALX1 in the presence of EP300.
• Involvement in diseaseNote=Chromosomal aberrations involving CREBBP may be a cause of acute myeloid leukemias. Translocation t(8;16)(p11;p13) with MYST3/MOZ; translocation t(11;16)(q23;p13.3) with MLL/HRX; translocation t(10;16)(q22;p13) with MYST4/MORF. MYST3-CREBBP may induce leukemia by inhibiting RUNX1-mediated transcription.
  Defects in CREBBP are a cause of Rubinstein-Taybi syndrome type 1 (RSTS1) [MIM:180849]. RSTS1 is an autosomal dominant disorder characterized by craniofacial abnormalities, broad thumbs, broad big toes, mental retardation and a propensity for development of malignancies.
• Sequence similaritiesContains 1 bromo domain.
  Contains 1 KIX domain.
  Contains 2 TAZ-type zinc fingers.
  Contains 1 ZZ-type zinc finger.
• DomainThe KIX domain mediates binding to HIV-1 Tat.
• Post-translational
  modificationsMethylation of the KIX domain by CARM1 blocks association with CREB. This results in the blockade of CREB signaling, and in activation of apoptotic response.
  Phosphorylated upon DNA damage, probably by ATM or ATR.
  Sumoylation negatively regulates transcriptional activity via the recruitment of DAAX.
• Cellular localizationCytoplasm. Nucleus. Recruited to nuclear bodies by SS18L1/CREST. In the presence of ALX1 relocalizes from the cytoplasm to the nucleus.

Target information above from: UniProt accession Q92793 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 1387 Human
  • Entrez Gene: 12914 Mouse
  • Omim: 600140 Human
  • SwissProt: Q92793 Human
  • SwissProt: P45481 Mouse
  • Unigene: 459759 Human
  • Unigene: 132238 Mouse
  • Unigene: 392384 Mouse

• Alternative names
  CBP antibodyCBP/p300 antibodyCBP_HUMAN antibody

  CREB binding protein antibodyCREB-binding protein antibodyCREBBP antibodyCyclic AMP responsive enhancer binding protein antibodyCyclic AMP-responsive enhancer binding protein antibodyKAT3A antibodyRSTS antibodyRTS antibodyRubinstein Taybi syndrome antibodyRubinstein-Taybi syndrome antibody

  see all

Anti-KAT3A / CBP antibody - ChIP Grade images

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  Immunoprecipitation - KAT3A / CBP antibody - ChIP Grade (ab10490)

  ab10490 immunoprecipitation of CREB Binding Protein.

  Samples: Nuclear (NE) or cytoplasmic (S100) extract (10 mg) from HeLa cells. Antibody: ab10490 used at 20 µg/10 mg extract. Detection: Coomassie Brilliant Blue R250 staining of SDS-PAGE gel followed by mass spectrometry to confirm the identity of the band that is CREB Binding Protein.

• 

  ChIP - CBP antibody - ChIP Grade (ab10490)

  Sonicated chromatin prepared from U2OS cells was subjected to the ChIP procedure with ab10490 to CBP. Immunoprecipitated chromatin was analysed in the promoter region of c-FOS (active) and in exon 2 of MYO-D (inactive), values are % of inputs.  2–4 µg of ab10490 and 1-2x10⁶ cells were used in each ChIP experiment.

  Sergei Denissov, Prof. Henk Stunnenberg lab, University Nijmengen, NL