Anti-KAT3B / p300 antibody [3G230 / NM-11] - ChIP Grade (ab14984)

Overview

• Product nameAnti-KAT3B / p300 antibody [3G230 / NM-11] - ChIP GradeSee all KAT3B / p300 primary antibodies ...
• Description
  Mouse monoclonal [3G230 / NM-11] to KAT3B / p300 - ChIP Grade
• Tested applicationsIP, IHC-P, ICC/IF, IHC-FoFr, WB, ChIP, IHC-Fr more details
• Species reactivity
  Reacts with: Human
  

  Does not react with

  Mouse, Rat
• Immunogen

  Recombinant full length protein (Human).

• Positive controlThis antibody gave a positive signal in HeLa and Jurkat whole cell lysates.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferpH: 7.40
  Preservative: 0.02% Sodium azide
  Constituent: PBS
• Concentration information loading...
• PurityProtein G purified
• Clonality Monoclonal
• Clone number3G230 / NM-11
• IsotypeIgG1
• Light chain typekappa
• Research Areas
  • Metabolism
  • Types of disease
  • Obesity

  • Metabolism
  • Pathways and Processes
  • Metabolism processes
  • Hypoxia

  • Metabolism
  • Types of disease
  • Cancer

  • Stem Cells
  • Signaling Pathways
  • TGF beta
  • Nuclear

  • Cancer
  • Oncoproteins/suppressors
  • Tumor suppressors
  • Rb family

  • Cancer
  • Cancer Metabolism
  • Response to hypoxia

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Other factors

  • Epigenetics and Nuclear Signaling
  • ChIP'ing antibodies
  • ChIP'ing antibodies

  • Epigenetics and Nuclear Signaling
  • Chromatin Modifying Enzymes
  • Acetylation

Applications

Target

• FunctionFunctions as histone acetyltransferase and regulates transcription via chromatin remodeling. Acetylates all four core histones in nucleosomes. Histone acetylation gives an epigenetic tag for transcriptional activation. Mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. Also functions as acetyltransferase for nonhistone targets. Acetylates 'Lys-131' of ALX1 and acts as its coactivator in the presence of CREBBP. Acetylates SIRT2 and is proposed to indirectly increase the transcriptional activity of TP53 through acetylation and subsequent attenuation of SIRT2 deacetylase function. Acetylates HDAC1 leading to its inactivation and modulation of transcription. Acts as a TFAP2A-mediated transcriptional coactivator in presence of CITED2. Plays a role as a coactivator of NEUROD1-dependent transcription of the secretin and p21 genes and controls terminal differentiation of cells in the intestinal epithelium. Promotes cardiac myocyte enlargement. Can also mediate transcriptional repression. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes. Acetylates FOXO1 and enhances its transcriptional activity.
• Involvement in diseaseNote=Defects in EP300 may play a role in epithelial cancer.
  Note=Chromosomal aberrations involving EP300 may be a cause of acute myeloid leukemias. Translocation t(8;22)(p11;q13) with KAT6A.
  Rubinstein-Taybi syndrome 2 (RSTS2) [MIM:613684]: A disorder characterized by craniofacial abnormalities, postnatal growth deficiency, broad thumbs, broad big toes, mental retardation and a propensity for development of malignancies. Some individuals with RSTS2 have less severe mental impairment, more severe microcephaly, and a greater degree of changes in facial bone structure than RSTS1 patients. Note=The disease is caused by mutations affecting the gene represented in this entry.
• Sequence similaritiesContains 1 bromo domain.
  Contains 1 KIX domain.
  Contains 2 TAZ-type zinc fingers.
  Contains 1 ZZ-type zinc finger.
• DomainThe CRD1 domain (cell cycle regulatory domain 1) mediates transcriptional repression of a subset of p300 responsive genes; it can be de-repressed by CDKN1A/p21WAF1 at least at some promoters. It conatins sumoylation and acetylation sites and the same lysine residues may be targeted for the respective modifications. It is proposed that deacetylation by SIRT1 allows sumoylation leading to suppressed activity.
• Post-translational
  modificationsAcetylated on Lys at up to 17 positions by intermolecular autocatalysis. Deacetylated in the transcriptional repression domain (CRD1) by SIRT1, preferentially at Lys-1020.
  Citrullinated at Arg-2142 by PADI4, which impairs methylation by CARM1 and promotes interaction with NCOA2/GRIP1.
  Methylated at Arg-580 and Arg-604 in the KIX domain by CARM1, which blocks association with CREB, inhibits CREB signaling and activates apoptotic response. Also methylated at Arg-2142 by CARM1, which impairs interaction with NCOA2/GRIP1.
  Sumoylated; sumoylation in the transcriptional repression domain (CRD1) mediates transcriptional repression. Desumoylated by SENP3 through the removal of SUMO2 and SUMO3.
  Probable target of ubiquitination by FBXO3, leading to rapid proteasome-dependent degradation.
  Phosphorylated by HIPK2 in a RUNX1-dependent manner. This phosphorylation that activates EP300 happens when RUNX1 is associated with DNA and CBFB. Phosphorylated by ROCK2 and this enhances its activity. Phosphorylation at Ser-89 by AMPK reduces interaction with nuclear receptors, such as PPARG.
• Cellular localizationCytoplasm. Nucleus. In the presence of ALX1 relocalizes from the cytoplasm to the nucleus. Co-localizes with ROCK2 in the nucleus.

Target information above from: UniProt accession Q09472 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 2033 Human
  • Omim: 602700 Human
  • SwissProt: Q09472 Human
  • Unigene: 517517 Human

• Alternative names
  CREBBP/EP300 inhibitory protein 1 antibodyCyclic AMP responsive enhancer binding protein antibodyE1A associated protein p300 antibody

  E1A binding protein p300 antibodyE1A-associated protein p300 antibodyEC 2.3.1.48 antibodyEP300 antibodyEP300: E1A binding protein p300 antibodyEP300_HUMAN antibodyHistone acetyltransferase p300 antibodyp300 HAT antibodyRB and P300 binding protein EID 1 antibodyRetinoblastoma protein associated protein antibodyRSTS2 antibody

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Anti-KAT3B / p300 antibody [3G230 / NM-11] - ChIP Grade images

• 

  ChIP - Anti-KAT3B / p300 antibody [3G230] - ChIP Grade (ab14984)

  ChIP was performed on HeLa chromatin from 4 million cells using 8ug of ab14984. Positive control locus IRS2 shows enrichment over the negative control MyoD as expected.

  This data was provided by an anonymous collaborator.

• 

  Western blot - Anti-KAT3B / p300 antibody [3G230] - ChIP Grade (ab14984)
  All lanes : Anti-KAT3B / p300 antibody [3G230 / NM-11] - ChIP Grade (ab14984) at 5 µg/ml
  
  Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
  Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Nuclear Lysate (ab14844)
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Predicted band size : 300 kDa
  Observed band size : 300 kDa
  Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.
  
  Exposure time : 20 minutes