Anti-KAT3B / p300 (phospho S89) antibody (ab111870)

Overview

• Product nameAnti-KAT3B / p300 (phospho S89) antibodySee all KAT3B / p300 primary antibodies ...
• Description
  Rabbit polyclonal to KAT3B / p300 (phospho S89)
• Specificityab111870 detects endogenous levels of KAT3B / p300 only when phosphorylated at serine 89.
• Tested applicationsIHC-P more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Rat
• Immunogen

  Synthetic phosphopeptide derived from Human KAT3B / p300 around the phosphorylation site of serine 89 (S-G-S^P-S-P).

• Positive controlHuman thyroid gland tissue.

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Storage bufferpH: 7.40
  Preservative: 0.02% Sodium azide
  Constituents: 49.1% PBS, 50% Glycerol, 0.88% Sodium chloride
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesab111870 was affinity-purified from Rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Types of disease
  • Obesity

  • Metabolism
  • Pathways and Processes
  • Metabolism processes
  • Hypoxia

  • Stem Cells
  • Signaling Pathways
  • TGF beta
  • Nuclear

  • Cancer
  • Cancer Metabolism
  • Response to hypoxia

  • Cancer
  • Oncoproteins/suppressors
  • Tumor suppressors
  • Rb family

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Other factors

  • Epigenetics and Nuclear Signaling
  • Chromatin Modifying Enzymes
  • Acetylation

Applications

Target

• FunctionFunctions as histone acetyltransferase and regulates transcription via chromatin remodeling. Acetylates all four core histones in nucleosomes. Histone acetylation gives an epigenetic tag for transcriptional activation. Mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. Also functions as acetyltransferase for nonhistone targets. Acetylates 'Lys-131' of ALX1 and acts as its coactivator in the presence of CREBBP. Acetylates SIRT2 and is proposed to indirectly increase the transcriptional activity of TP53 through acetylation and subsequent attenuation of SIRT2 deacetylase function. Acetylates HDAC1 leading to its inactivation and modulation of transcription. Acts as a TFAP2A-mediated transcriptional coactivator in presence of CITED2. Plays a role as a coactivator of NEUROD1-dependent transcription of the secretin and p21 genes and controls terminal differentiation of cells in the intestinal epithelium. Promotes cardiac myocyte enlargement. Can also mediate transcriptional repression. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes. Acetylates FOXO1 and enhances its transcriptional activity.
• Involvement in diseaseNote=Defects in EP300 may play a role in epithelial cancer.
  Note=Chromosomal aberrations involving EP300 may be a cause of acute myeloid leukemias. Translocation t(8;22)(p11;q13) with KAT6A.
  Rubinstein-Taybi syndrome 2 (RSTS2) [MIM:613684]: A disorder characterized by craniofacial abnormalities, postnatal growth deficiency, broad thumbs, broad big toes, mental retardation and a propensity for development of malignancies. Some individuals with RSTS2 have less severe mental impairment, more severe microcephaly, and a greater degree of changes in facial bone structure than RSTS1 patients. Note=The disease is caused by mutations affecting the gene represented in this entry.
• Sequence similaritiesContains 1 bromo domain.
  Contains 1 KIX domain.
  Contains 2 TAZ-type zinc fingers.
  Contains 1 ZZ-type zinc finger.
• DomainThe CRD1 domain (cell cycle regulatory domain 1) mediates transcriptional repression of a subset of p300 responsive genes; it can be de-repressed by CDKN1A/p21WAF1 at least at some promoters. It conatins sumoylation and acetylation sites and the same lysine residues may be targeted for the respective modifications. It is proposed that deacetylation by SIRT1 allows sumoylation leading to suppressed activity.
• Post-translational
  modificationsAcetylated on Lys at up to 17 positions by intermolecular autocatalysis. Deacetylated in the transcriptional repression domain (CRD1) by SIRT1, preferentially at Lys-1020.
  Citrullinated at Arg-2142 by PADI4, which impairs methylation by CARM1 and promotes interaction with NCOA2/GRIP1.
  Methylated at Arg-580 and Arg-604 in the KIX domain by CARM1, which blocks association with CREB, inhibits CREB signaling and activates apoptotic response. Also methylated at Arg-2142 by CARM1, which impairs interaction with NCOA2/GRIP1.
  Sumoylated; sumoylation in the transcriptional repression domain (CRD1) mediates transcriptional repression. Desumoylated by SENP3 through the removal of SUMO2 and SUMO3.
  Probable target of ubiquitination by FBXO3, leading to rapid proteasome-dependent degradation.
  Phosphorylated by HIPK2 in a RUNX1-dependent manner. This phosphorylation that activates EP300 happens when RUNX1 is associated with DNA and CBFB. Phosphorylated by ROCK2 and this enhances its activity. Phosphorylation at Ser-89 by AMPK reduces interaction with nuclear receptors, such as PPARG.
• Cellular localizationCytoplasm. Nucleus. In the presence of ALX1 relocalizes from the cytoplasm to the nucleus. Co-localizes with ROCK2 in the nucleus.

Target information above from: UniProt accession Q09472 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 2033 Human
  • Entrez Gene: 328572 Mouse
  • Entrez Gene: 170915 Rat
  • Omim: 602700 Human
  • SwissProt: Q09472 Human
  • SwissProt: B2RWS6 Mouse
  • Unigene: 517517 Human
  • Unigene: 258397 Mouse

  • Unigene: 12447 Rat

  see all

• Alternative names
  CREBBP/EP300 inhibitory protein 1 antibodyCyclic AMP responsive enhancer binding protein antibodyE1A associated protein p300 antibody

  E1A binding protein p300 antibodyE1A-associated protein p300 antibodyEC 2.3.1.48 antibodyEP300 antibodyEP300: E1A binding protein p300 antibodyEP300_HUMAN antibodyHistone acetyltransferase p300 antibodyp300 HAT antibodyRB and P300 binding protein EID 1 antibodyRetinoblastoma protein associated protein antibodyRSTS2 antibody

  see all

Anti-KAT3B / p300 (phospho S89) antibody images

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - KAT3B / p300 (phospho S89) antibody (ab111870)
  ab111870 at 1/50 dilution staining KAT3B / p300 in paraffin-embedded Human Thyroid gland tissue by Immunohistochemistry. The image on the right is treated with the synthesized phosphopeptide.