Anti-KAT4 / TBP Associated Factor 1 antibody - ChIP Grade (ab28450)

Overview

• Product nameAnti-KAT4 / TBP Associated Factor 1 antibody - ChIP GradeSee all KAT4 / TBP Associated Factor 1 primary antibodies ...
• Description
  Rabbit polyclonal to KAT4 / TBP Associated Factor 1 - ChIP Grade
• Specificityab28450 recognises transcription initiation factor TFIID subunit 1 (TAF1).
• Tested applicationsIHC-P, ELISA, WB, ChIP more details
• Species reactivity
  Reacts with: Mouse, Human
  Predicted to work with: Rat, Dog
• Immunogen

  Synthetic peptide derived from residues 1823 - 1872 (C-terminal) of human TAF1.

• Positive controlDaudi cell lysate, Human kidney tissue.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: 2% Sucrose, PBS
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Epigenetics and Nuclear Signaling
  • Transcription
  • Polymerase associated factors
  • Pol II Transcription
  • TFIID

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Co-factors

  • Epigenetics and Nuclear Signaling
  • ChIP'ing antibodies
  • ChIP'ing antibodies

  • Epigenetics and Nuclear Signaling
  • Chromatin Modifying Enzymes
  • Acetylation
  • HAT

Applications

Target

• FunctionLargest component and core scaffold of the TFIID basal transcription factor complex. Contains novel N- and C-terminal Ser/Thr kinase domains which can autophosphorylate or transphosphorylate other transcription factors. Phosphorylates TP53 on 'Thr-55' which leads to MDM2-mediated degradation of TP53. Phosphorylates GTF2A1 and GTF2F1 on Ser residues. Possesses DNA-binding activity. Essential for progression of the G1 phase of the cell cycle.
• Involvement in diseaseDefects in TAF1 are the cause of dystonia type 3 (DYT3) [MIM:314250]; also called X-linked dystonia-parkinsonism (XDP). DYT3 is a X-linked dystonia-parkinsonism disorder. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. DYT3 is characterized by severe progressive torsion dystonia followed by parkinsonism. Its prevalence is high in the Philippines. DYT3 has a well-defined pathology of extensive neuronal loss and mosaic gliosis in the striatum (caudate nucleus and putamen) which appears to resemble that in Huntington disease.
• Sequence similaritiesBelongs to the TAF1 family.
  Contains 2 bromo domains.
  Contains 1 HMG box DNA-binding domain.
  Contains 2 protein kinase domains.
• Post-translational
  modificationsPhosphorylated by casein kinase II in vitro.
• Cellular localizationNucleus.

Target information above from: UniProt accession P21675 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 6872 Human
  • Entrez Gene: 270627 Mouse
  • Entrez Gene: 317256 Rat
  • Omim: 313650 Human
  • SwissProt: P21675 Human
  • SwissProt: Q80UV9 Mouse
  • Unigene: 158560 Human
  • Unigene: 722619 Human

  • Unigene: 261750 Mouse

  see all

• Alternative names
  BA2R antibodyCCG 1 antibodyCCG1 antibody

  CCGS antibodyCell cycle G1 phase defect antibodyCell cycle gene 1 protein antibodyComplementation of cell cycle block G1 to S antibodyDYT3 antibodyDYT3 protein antibodyN TAF1 antibodyNSCL 2 antibodyNSCL2 antibodyOF antibodyp250 antibodyTAF 1 antibodyTAF 2A antibodyTAF(II)250 antibodyTAF1 antibodyTAF1 RNA polymerase II TATA box binding protein (TBP) associated factor 250kDa antibodyTAF1 RNA polymerase II TATA box binding protein (TBP) associated factor antibodyTAF1 RNA polymerase II TATA box binding protein associated factor 250kDa antibodyTAF1_HUMAN antibodyTAF2A antibodyTAFII 250 antibodyTAFII-250 antibodyTAFII250 antibodyTATA box binding protein (TBP) associated factor RNA polymerase II A antibodyTATA box binding protein (TBP) associated factor RNA polymerase II A 250kD antibodyTBP associated factor 250 kDa antibodyTBP-associated factor 250 kDa antibodyTranscription factor TFIID p250 polypeptide antibodyTranscription initiation factor TFIID 250 kDa subunit antibodyTranscription initiation factor TFIID subunit 1 antibodyXDP antibody

  see all

Anti-KAT4 / TBP Associated Factor 1 antibody - ChIP Grade images

• 

  Immunohistochemistry (Paraffin-embedded sections) - TAF1 antibody (ab28450)
  This image shows human kidney stained with ab28450 at 8µg/ml. Magnification 400x.
• 

  Western blot - TAF1 antibody (ab28450)
  Anti-KAT4 / TBP Associated Factor 1 antibody - ChIP Grade (ab28450) at 0.5 µg/ml + Daudi cell lysate
  
  Predicted band size : 212 kDa
  
• 

  ChIP - TAF1 antibody - ChIP Grade (ab28450)

  Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10mins. The  ChIP was performed with 25µg of chromatin, 8µg of  ab28450 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman and sybr green approach). Primers and probes for the actively transcribed genes GAPDH and ACT1 are located in the core promoter.