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Products:Epigenetics and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol II Transcription >> TFIID
Overview
Product name
Anti-KAT4 / TBP Associated Factor 1 antibody [0.T.145]
See all KAT4 / TBP Associated Factor 1 products (7) ...
Description
Mouse monoclonal [0.T.145] to KAT4 / TBP Associated Factor 1
Tested applications
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Full length protein (Human).
Positive control
HeLa nuclear lysate.
General notes
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.035% Sodium Azide
Constituents: 30% Glycerol, 0.105M Sodium Chloride, 0.07M Tris-glycine. pH 7.4
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Clone number
0.T.145
Isotype
IgG1
Light chain type
kappa
Applications
Show applications key
Our Abpromise guarantee covers the use of ab14211 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 250 kDa (predicted molecular weight: 227 kDa).
Target
Function
Largest component and core scaffold of the TFIID basal transcription factor complex. Contains novel N- and C-terminal Ser/Thr kinase domains which can autophosphorylate or transphosphorylate other transcription factors. Phosphorylates TP53 on 'Thr-55' which leads to MDM2-mediated degradation of TP53. Phosphorylates GTF2A1 and GTF2F1 on Ser residues. Possesses DNA-binding activity. Essential for progression of the G1 phase of the cell cycle.
Involvement in disease
Defects in TAF1 are the cause of dystonia type 3 (DYT3) [MIM:314250]; also called X-linked dystonia-parkinsonism (XDP). DYT3 is a X-linked dystonia-parkinsonism disorder. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. DYT3 is characterized by severe progressive torsion dystonia followed by parkinsonism. Its prevalence is high in the Philippines. DYT3 has a well-defined pathology of extensive neuronal loss and mosaic gliosis in the striatum (caudate nucleus and putamen) which appears to resemble that in Huntington disease.
Sequence similarities
Belongs to the TAF1 family.
Contains 2 bromo domains.
Contains 1 HMG box DNA-binding domain.
Contains 2 protein kinase domains.
Post-translational
modifications
Phosphorylated by casein kinase II in vitro.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP21675
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BA2R antibody
- CCG1 antibody
- CCGS antibody
see all
Database links
- Entrez Gene: 6872 Human
- Entrez Gene: 270627 Mouse
- Entrez Gene: 317256 Rat
- Omim: 313650 Human
- SwissProt: P21675 Human
- SwissProt: Q80UV9 Mouse
- Unigene: 1179 Human
- Unigene: 158560 Human
see all
References for Anti-KAT4 / TBP Associated Factor 1 antibody [0.T.145] (ab14211)
ab14211 has not yet been referenced specifically in any publications.
Publishing research using ab14211? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"