|
ab46666 |
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab28450? Please let us know so that we can cite the reference in this datasheet
ab28450 has been referenced in 2 publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
This image shows human kidney stained with ab28450 at 8µg/ml. Magnification 400x.
Anti-KAT4 / TBP Associated Factor 1 antibody - ChIP Grade (ab28450) at 0.5 µg/ml + Daudi cell lysate
Predicted band size : 212 kDa
Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10mins. The ChIP was performed with 25µg of chromatin, 8µg of ab28450 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman and sybr green approach). Primers and probes for the actively transcribed genes GAPDH and ACT1 are located in the core promoter.
0
Call 01223 696 000 or contact us