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Overview
Product name
Anti-KAT4 / TBP Associated Factor 1 antibody - ChIP Grade
See all KAT4 / TBP Associated Factor 1 products (7) ...
Description
Rabbit polyclonal to KAT4 / TBP Associated Factor 1 - ChIP Grade
Specificity
ab28450 recognises transcription initiation factor TFIID subunit 1 (TAF1).
Tested applications
IHC-P, ELISA, WB, ChIPmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Rat, Dog
Immunogen
Synthetic peptide derived from residues 1823 - 1872 (C-terminal) of human TAF1.
Positive control
Daudi cell lysate, Human kidney tissue.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HAT
Epigenetics and Nuclear Signaling >> Transcription >> Co-factors
Epigenetics and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol II Transcription >> TFIID
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation
Applications
Show applications key
Our Abpromise guarantee covers the use of ab28450 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 4-8 µg/ml
ELISA
ELISA: 1/62500ELISA: 1/62500
WB: Use a concentration of 0.5 µg/mlPredicted molecular weight: 212 kDa.
ChIP: Use 5-10 µg for 25 µg of chromatin.
Target
Function
Largest component and core scaffold of the TFIID basal transcription factor complex. Contains novel N- and C-terminal Ser/Thr kinase domains which can autophosphorylate or transphosphorylate other transcription factors. Phosphorylates TP53 on 'Thr-55' which leads to MDM2-mediated degradation of TP53. Phosphorylates GTF2A1 and GTF2F1 on Ser residues. Possesses DNA-binding activity. Essential for progression of the G1 phase of the cell cycle.
Involvement in disease
Defects in TAF1 are the cause of dystonia type 3 (DYT3) [MIM:314250]; also called X-linked dystonia-parkinsonism (XDP). DYT3 is a X-linked dystonia-parkinsonism disorder. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. DYT3 is characterized by severe progressive torsion dystonia followed by parkinsonism. Its prevalence is high in the Philippines. DYT3 has a well-defined pathology of extensive neuronal loss and mosaic gliosis in the striatum (caudate nucleus and putamen) which appears to resemble that in Huntington disease.
Sequence similarities
Belongs to the TAF1 family.
Contains 2 bromo domains.
Contains 1 HMG box DNA-binding domain.
Contains 2 protein kinase domains.
Post-translational
modifications
Phosphorylated by casein kinase II in vitro.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP21675
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BA2R antibody
- CCG 1 antibody
- CCG1 antibody
see all
Database links
- Entrez Gene: 6872 Human
- Entrez Gene: 270627 Mouse
- Entrez Gene: 317256 Rat
- Omim: 313650 Human
- SwissProt: P21675 Human
- SwissProt: Q80UV9 Mouse
- Unigene: 1179 Human
- Unigene: 158560 Human
see all
Anti-KAT4 / TBP Associated Factor 1 antibody - ChIP Grade images:
Immunohistochemistry (Paraffin-embedded sections) - TAF1 antibody (ab28450)
This image shows human kidney stained with ab28450 at 8µg/ml. Magnification 400x.
Western blot - TAF1 antibody (ab28450)
Anti-KAT4 / TBP Associated Factor 1 antibody - ChIP Grade (ab28450) at 0.5 µg/ml + Daudi cell lysate
Predicted band size : 212 kDa
ChIP - TAF1 antibody - ChIP Grade (ab28450)
Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10mins. The ChIP was performed with 25µg of chromatin, 8µg of ab28450 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman and sybr green approach). Primers and probes for the actively transcribed genes GAPDH and ACT1 are located in the core promoter.
References for Anti-KAT4 / TBP Associated Factor 1 antibody - ChIP Grade (ab28450)
This product has been referenced in:
- Iwata Jet al. Transforming growth factor-beta regulates basal transcriptional regulatory machinery to control cell proliferation and differentiation in cranial neural crest-derived osteoprogenitor cells. J Biol Chem 285:4975-82 (2010). WB; Mouse.Read more (PubMed: 19959467) »
- van Essen Det al. Two modes of transcriptional activation at native promoters by NF-kappaB p65. PLoS Biol 7:e73 (2009). ChIP; Mouse.Read more (PubMed: 19338389) »
See all 2 publications for this product
Publishing research using ab28450? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"