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Overview
Product name
Anti-KAT4 / TBP Associated Factor 1 antibody
See all KAT4 / TBP Associated Factor 1 products (7) ...
Description
Mouse polyclonal to KAT4 / TBP Associated Factor 1
Tested applications
Cross reactivity
Reacts with
Saccharomyces cerevisiae
Immunogen
Fusion protein: LSNDDKYQILKKTHQTKVRSTISNLNIQHSQPAINLQSPF YKVAVPRYQLRHFHRENFGSHIRPGTKIVFSKLKARKRKR DKGKDVKESFSTSQDLTIGD, corresponding to amino acids 441/540 of Saccharomyces cerevisiae TAF1
LSNDDKYQIL KKTHQTKVRS TISNLNIQHS QPAINLQSPF YKVAVPRYQL RHFHRENFGS HIRPGTKIVF SKLKARKRKR DKGKDVKESF STSQDLTIGD
General notes
Produced from outbred CD1 mice
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 50% Glycerol
Purity
Whole antiserum
Primary antibody notes
This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al.PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HAT
Epigenetics and Nuclear Signaling >> Transcription >> Co-factors
Epigenetics and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol II Transcription >> TFIID
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation
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Western blot - TAF1 antibody (ab24427)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab24427 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: 1/1000. Predicted molecular weight: 120 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.
Target
Function
Largest component and core scaffold of the TFIID basal transcription factor complex. Contains novel N- and C-terminal Ser/Thr kinase domains which can autophosphorylate or transphosphorylate other transcription factors. Phosphorylates TP53 on 'Thr-55' which leads to MDM2-mediated degradation of TP53. Phosphorylates GTF2A1 and GTF2F1 on Ser residues. Possesses DNA-binding activity. Essential for progression of the G1 phase of the cell cycle.
Involvement in disease
Defects in TAF1 are the cause of dystonia type 3 (DYT3) [MIM:314250]; also called X-linked dystonia-parkinsonism (XDP). DYT3 is a X-linked dystonia-parkinsonism disorder. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. DYT3 is characterized by severe progressive torsion dystonia followed by parkinsonism. Its prevalence is high in the Philippines. DYT3 has a well-defined pathology of extensive neuronal loss and mosaic gliosis in the striatum (caudate nucleus and putamen) which appears to resemble that in Huntington disease.
Sequence similarities
Belongs to the TAF1 family.
Contains 2 bromo domains.
Contains 1 HMG box DNA-binding domain.
Contains 2 protein kinase domains.
Post-translational
modifications
Phosphorylated by casein kinase II in vitro.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP21675
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BA2R antibody
- CCG 1 antibody
- CCG1 antibody
see all
Database links
- Entrez Gene: 853191 Saccharomyces cerevisiae
- SwissProt: P46677 Saccharomyces cerevisiae
Anti-KAT4 / TBP Associated Factor 1 antibody images:
Western blot - TAF1 antibody (ab24427)
All lanes : Anti-KAT4 / TBP Associated Factor 1 antibody (ab24427) at 1/1000 dilution
Lane 1 : Total protein extract from E. coli with ~50ng to 100ng of a negative control fusion protein with an irrelevant antigen at 20 ug
Lane 2 : Total protein extract from E. coli with ~50ng to 500ng of the antigen fusion protein at 20 ug
Secondary
Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution
Predicted band size : 120 kDa
The molecular weight of the band on the western blot does not correspond to the predicted band size above (predicted from the molecular weight of the natural protein) because of the additional mass of the fusion and because the fusion protein only contains a partial fragment of the gene.
References for Anti-KAT4 / TBP Associated Factor 1 antibody (ab24427)
ab24427 has not yet been referenced specifically in any publications.
Publishing research using ab24427? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"