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Western blot - TAF1 antibody (ab24427)
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Product Name
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KAT4 / TBP Associated Factor 1 antibody
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See all KAT4 / TBP Associated Factor 1 antibodies (7)...
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Product type
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Primary antibodies
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Description
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Mouse polyclonal to KAT4 / TBP Associated Factor 1
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Immunogen
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Fusion protein: LSNDDKYQIL KKTHQTKVRS TISNLNIQHS QPAINLQSPF YKVAVPRYQL RHFHRENFGS HIRPGTKIVF SKLKARKRKR DKGKDVKESF STSQDLTIGD corresponding to amino acids 441/540 of Saccharomyces cerevisiae TAF1
BLAST 'LSNDDKYQIL KKTHQTKVRS TISNLNIQHS QPAINLQSPF YKVAVPRYQL RHFHRENFGS HIRPGTKIVF SKLKARKRKR DKGKDVKESF STSQDLTIGD ' with  or 
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Reacts with
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Sc
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Tested applications
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: 1/1000. Predicted molecular weight: 120 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HAT
Chromatin and Nuclear Signaling >> Transcription >> Co-factors
Chromatin and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol II Transcription >> TFIID
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Relevance
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KAT4 / TAF1 also known as TBP Associated Factor 1 is the largest subunit of the transcription factor TFIID. Studies have shown that KAT4 has serine/threonine domains that can autophosphorylate the large subunit of TFIIF. More recent studies have shown that it is also a histone acetylytransferase, suggesting that transcriptional co activators may also have a role in targeting acetylation activity to promoters.
This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein.
A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein.
Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
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KAT4 / TBP Associated Factor 1 antibody (ab24427) |
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Raised in
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Mouse
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Whole antiserum
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Storage buffer
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Preservative: None
Constituents: 50% Glycerol Material safety datasheet (MSDS) for this product:
Glycerol MSDS
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
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Notes
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Produced from outbred CD1 mice
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this KAT4 / TBP Associated Factor 1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for KAT4 / TBP Associated Factor 1 antibody images, references, products related to ab24427 and other tools.
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KAT4 / TBP Associated Factor 1 antibody images:
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 Western blot - TAF1 antibody (ab24427)
All lanes : KAT4 / TBP Associated Factor 1 antibody (ab24427) at 1/1000 dilution
Lane 1 : Total protein extract from E. coli with ~50ng to 100ng of a negative control fusion protein with an irrelevant antigen at 20 ug
Lane 2 : Total protein extract from E. coli with ~50ng to 500ng of the antigen fusion protein at 20 ug
Secondary
Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution
Predicted band size : 120 kDa
The molecular weight of the band on the western blot does not correspond to the predicted band size above (predicted from the molecular weight of the natural protein) because of the additional mass of the fusion and because the fusion protein only contains a partial fragment of the gene.
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Search PubMed (MEDLINE) for references to KAT4 / TBP Associated Factor 1
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