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Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human Tip60.
(Peptide available as ab26349.)
Our Abpromise guarantee covers the use of ab23886 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
|ICC/IF||Use a concentration of 1 µg/ml.|
ab23886 staining KAT5 / Tip60 in human primary fibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.2% Triton X-100 and blocked with 2% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/500 in PBS + 2% BSA) for 2 hours. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.