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- Secondary antibodies
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Product name
Anti-KAT7 / Hbo1 / MYST2 antibody
See all KAT7 / Hbo1 / MYST2 products (5) ...
Description
Rabbit polyclonal to KAT7 / Hbo1 / MYST2
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Chicken, Cow, Dog, Pig
Immunogen
Synthetic peptide corresponding to a region within amino acids 2-51 (PRRKRNAGSS SDGTEDSDFS TDLEHTDSSE SDGTSRRSAR VTRSSARLSQ) of Human KAT7 / Hbo1 / MYST2 (NP_008998).
PRRKRNAGSSSDGTEDSDFSTDLEHTDSSESDGTSRRSAR VTRSSARLSQ
Positive control
OVCAR-3 cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Histones >> H4 >> Acetylated
Epigenetics and Nuclear Signaling >> Histones >> H3 >> Acetylated
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation
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Western blot - KAT7 / Hbo1 / MYST2 antibody (ab87100)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab87100 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/ml. Predicted molecular weight: 71 kDa.Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Target
Function
Component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. Through chromatin acetylation it may regulate DNA replication and act as a coactivator of TP53-dependent transcription. Specifically represses AR-mediated transcription.
Tissue specificity
Ubiquitously expressed, with highest levels in testis.
Sequence similarities
Belongs to the MYST (SAS/MOZ) family.
Contains 1 C2HC-type zinc finger.
Domain
The C2HC-type zinc finger is required for interaction with MCM2 and ORC1L.
The N-terminus is involved in transcriptional repression, while the C-terminus mediates AR-interaction.
Post-translational
modifications
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization
Nucleus > nucleoplasm.
Target information above from: UniProt accessionO95251
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- SAS2 and TIP60 protein 2 antibody
- Hbo 1 antibody
- HBO1 antibody
see all
Database links
- Entrez Gene: 11143 Human
- Entrez Gene: 217127 Mouse
- Entrez Gene: 303470 Rat
- Omim: 609880 Human
- SwissProt: O95251 Human
- SwissProt: Q5SVQ0 Mouse
- SwissProt: Q810T5 Rat
- Unigene: 21907 Human
see all
Anti-KAT7 / Hbo1 / MYST2 antibody images:
Western blot - KAT7 / Hbo1 / MYST2 antibody (ab87100)
Anti-KAT7 / Hbo1 / MYST2 antibody (ab87100) at 1 µg/ml (in 5% skim milk / PBS buffer) + 10 µg OVCAR-3 cell lysate
Secondary
HRP conjugated anti-Rabbit IgG diluted 1/50,000.
Predicted band size : 71 kDa
Observed band size : 65 kDa (why is the actual band size different from the predicted?)
Additional bands at : 40 kDa,50 kDa. We are unsure as to the identity of these extra bands.
Gel concentration 12%
References for Anti-KAT7 / Hbo1 / MYST2 antibody (ab87100)
ab87100 has not yet been referenced specifically in any publications.
Publishing research using ab87100? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"