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Western blot - KBTBD10 antibody (ab66605)
Western blot - KBTBD10 antibody (ab66605)
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Product Name 

KBTBD10 antibody

 

See all KBTBD10 antibodies (2)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to KBTBD10

Immunogen 

Synthetic peptide: AGSLYAIGGF AMIQLESKEF APTEVNDIWK YEDDKKEWAG MLKEIRYASG, corresponding to a region within C terminal amino acids 541-590 of Human KBTBD10
BLAST 'AGSLYAIGGF AMIQLESKEF APTEVNDIWK YEDDKKEWAG MLKEIRYASG ' with BLAST the sequence with ExPASy or BLAST the sequence with NCBI

Reacts with 
(species key)

Hu, Ms, Rat, Dog

Tested applications 
(see key)

ELISA, WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
ELISA: Use at an assay dependent dilution.
titre using peptide based assay: 1:1562500

WB: Use at a concentration of 1.25 µg/ml. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Fetal muscle lysate

Cellular localization 

Cytoplasmic

Research areas 

Cell Biology >> Other Antibodies >> Other Antibodies
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ub-like Proteins

Relevance 

Sarcosin contains 1 BTB (POZ) domain and is required for pseudopod elongation in transformed cells. Sarcosin mRNA is up-regulated by less than two folds in the heart in human patients with HCM.

 

Alternative names KBTBD10 antibody (ab66605)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

10324    (Human)
228003    (Mouse)
117537    (Rat)

SwissProt

    

O60662    (Human)
A2AUC9    (Mouse)
Q9ER30    (Rat)

Unigene

    

50550    (Human)
28875    (Rat)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Protein A purified

Storage buffer 

Preservative: None
Constituents: 2% Sucrose, PBS

Form 

Lyophilised
Add 100 µl of distilled water.

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this KBTBD10 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for KBTBD10 antibody images, references, products related to ab66605 and other tools.

 

KBTBD10 antibody images:

Product image  Western blot - KBTBD10 antibody (ab66605)

Western blot - KBTBD10 antibody (ab66605)

KBTBD10 antibody (ab66605) at 1.25 µg/ml (in 5% skim milk / PBS buffer) + fetal muscle lysate at 10 µg

Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution

Predicted band size : 68 kDa
Observed band size : 68 kDa


gel concentration: 12%

 
 

Search PubMed (MEDLINE) for references to KBTBD10

 

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Other KBTBD10 Antibodies

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KBTBD10 antibody - other tools:

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