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Products:Neuroscience >> Neurotransmission >> Receptors / Channels >> Potassium Channels
Overview
Product name
Anti-KCNAB3 antibody
See all KCNAB3 products (2) ...
Description
Rabbit polyclonal to KCNAB3
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Cow, Dog
Immunogen
A region within synthetic peptide: GRGTGMKYRN LGKSGLRVSC LGLGTWVTFG SQISDETAED VLTVAYEHGV, corresponding to N terminal amino acids 73-122 of Human KCNAB3
GRGTGMKYRN LGKSGLRVSC LGLGTWVTFG SQISDETAED VLTVAYEHGV
Positive control
Human kidney tissue, Jurkat cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Neurotransmission >> Receptors / Channels >> Potassium Channels
Applications
Show applications key
Our Abpromise guarantee covers the use of ab66625 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
ELISA titre using peptide based assay 1:12500.
IHC-P: Use at a concentration of 4 - 8 µg/ml.
WB: Use at a concentration of 0.65 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Accessory potassium channel protein which modulates the activity of the pore-forming alpha subunit. Alters the functional properties of Kv1.5.
Tissue specificity
Brain specific. Most prominent expression in cerebellum. Weaker signals detected in cortex, occipital lobe, frontal lobe and temporal lobe. Not detected in spinal cord, heart, lung, liver, kidney, pancreas, placenta and skeletal muscle.
Sequence similarities
Belongs to the shaker potassium channel beta subunit family.
Domain
Alteration of functional properties of alpha subunit is mediated through N-terminal domain of beta subunit.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionO43448
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AKR6A9 antibody
- K antibody
- K(+) channel subunit beta-3 antibody
see all
Database links
- Entrez Gene: 789104 Cow
- Entrez Gene: 489483 Dog
- Entrez Gene: 9196 Human
- Entrez Gene: 16499 Mouse
- Entrez Gene: 58981 Rat
- Omim: 604111 Human
- SwissProt: O43448 Human
- SwissProt: P97382 Mouse
see all
Anti-KCNAB3 antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - KCNAB3 antibody (ab66625)
This image shows human kidney tissue stained with ab66625 at 4-8µg/ml. The arrows (positive label) point to epithelial cells of renal tubules.
Western blot - KCNAB3 antibody (ab66625)
Anti-KCNAB3 antibody (ab66625) at 0.65 µg/ml + Jurkat cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 44 kDa
Observed band size : 44 kDa
Additional bands at : 34 kDa. We are unsure as to the identity of these extra bands.
References for Anti-KCNAB3 antibody (ab66625)
ab66625 has not yet been referenced specifically in any publications.
Publishing research using ab66625? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"