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- Secondary antibodies
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Product name
Anti-KCNA3 antibody
See all KCNA3 products (6) ...
Description
Rabbit polyclonal to KCNA3
Specificity
This antibody recognizes full length KCNA3 protein. It does not cross react with other Kv1 channels.
Tested applications
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Tagged recombinant fragment: ARSNSTLSKS EYMVIEEGGM NQSAFPQTPF KTGNSTATCT TNNNPNSCVN IKK, corresponding to amino acids 471-523 of Mouse KCNA3
ARSNSTLSKSEYMVIEEGGMNQSAFPQTPFKTGNSTATCT TNNNPNSCVNIKK
Positive control
KCNA3 tagged control protein.
Properties
Form
Lyophilised:
Please reconstitute this product with 50ul deionized water.
Further dilutions should be made in a buffer with BSA (1-3%). To minimize aspecific staining, centrifuge antibody prep at 10,000g for 5min immediately before use to remove any aggregates.
POSITIVE CONTROL: Reconstitute control protein with 100µl dH2O. Then aliquot and freeze (-20°C). Use as positive control: 20 ng control protein per lane. Use as negative control: preincubate 3-5 ug control protein with 1 ug antibody for 1h at RT.
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 5% Sucrose, 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Metabolism >> Types of disease >> Cancer
Signal Transduction >> Metabolism >> Plasma Membrane >> Channels
Applications
Show applications key
Our Abpromise guarantee covers the use of ab54526 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
Application notes
WB: Use at a concentration of 0.75 - 1.5 µg/ml (or 1:200-1:400, chemiluminescence). Predicted molecular weight: 58 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient.
Sequence similarities
Belongs to the potassium channel family. A (Shaker) (TC 1.A.1.2) subfamily. Kv1.3/KCNA3 sub-subfamily.
Domain
The N-terminus may be important in determining the rate of inactivation of the channel while the tail may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.
The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
Cellular localization
Membrane.
Target information above from: UniProt accessionP22001
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- HGK 5 antibody
- HGK5 antibody
- HLK 3 antibody
see all
Database links
- Entrez Gene: 3738 Human
- Entrez Gene: 16491 Mouse
- Entrez Gene: 29731 Rat
- Omim: 176263 Human
- SwissProt: P22001 Human
- SwissProt: P16390 Mouse
- SwissProt: P15384 Rat
- Unigene: 169948 Human
see all
References for Anti-KCNA3 antibody (ab54526)
This product has been referenced in:
- Wang FE et al. MicroRNA-204/211 alters epithelial physiology. FASEB J : (2010).Read more (PubMed: 20056717) »
See 1 publication for this product
Publishing research using ab54526? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"