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Products:Signal Transduction >> Metabolism >> Plasma Membrane >> Channels
Overview
Product name
Anti-KCNA3 antibody
See all KCNA3 products (6) ...
Description
Rabbit polyclonal to KCNA3
Specificity
ab61200 detects endogenous levels of total KCNA3 protein.
Tested applications
ELISA, ICC/IF, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic non-phosphopeptide derived from human KCNA3 around the phosphorylation site of tyrosine 135.
Positive control
Human brain tissue and HuvEc cells.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab61200 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Metabolism >> Plasma Membrane >> Channels
Applications
Show applications key
Our Abpromise guarantee covers the use of ab61200 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/20000ELISA: 1/20000
ICC/IF: 1/100 - 1/500.
IHC-P: 1/50 - 1/100.
Target
Function
Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient.
Sequence similarities
Belongs to the potassium channel family. A (Shaker) (TC 1.A.1.2) subfamily. Kv1.3/KCNA3 sub-subfamily.
Domain
The N-terminus may be important in determining the rate of inactivation of the channel while the tail may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.
The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
Cellular localization
Membrane.
Target information above from: UniProt accessionP22001
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- HGK 5 antibody
- HGK5 antibody
- HLK 3 antibody
see all
Database links
- Entrez Gene: 3738 Human
- Entrez Gene: 16491 Mouse
- Omim: 176263 Human
- SwissProt: P22001 Human
- SwissProt: P16390 Mouse
- Unigene: 169948 Human
- Unigene: 30640 Mouse
Anti-KCNA3 antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - KCNA3 antibody (ab61200)
ab61200 at 1/50 dilution staining KCNA3 in human brain by Immunohistochemistry, Paraffin-embedded tissue, in the absence (left) or presence (right) of the immunising peptide.
Immunocytochemistry/ Immunofluorescence - KCNA3 antibody (ab61200)
ab61200 at 1/100 dilution staining KCNA3 in HuvEc cells by Immunofluorescence, in the absence (left) or presence (right) of the immunising peptide.
References for Anti-KCNA3 antibody (ab61200)
ab61200 has not yet been referenced specifically in any publications.
Publishing research using ab61200? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"