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Products:Neuroscience >> Neurotransmission >> Receptors / Channels >> Potassium Channels
Overview
Product name
Anti-KCNC4 antibody
See all KCNC4 products (5) ...
Description
Rabbit polyclonal to KCNC4
Specificity
Detects endogenous levels of total KCNC4 protein.
Tested applications
ELISA, WB, IHC-P, ICC/IFmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse
Immunogen
Synthesized non phosphopeptide derived from Human KCNC4 around the phosphorylation site of serine 15 (R-K-SP-G-N)
Positive control
Human brain tissue; extracts from COS7 cells treated with Anisomycin (25 µg/ml, 30 mins); HeLa cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
Neuroscience >> Neurotransmission >> Receptors / Channels >> Potassium Channels
Applications
Show applications key
Our Abpromise guarantee covers the use of ab79173 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/20000ELISA: 1/20000
WB: 1/500 - 1/1000.Predicted molecular weight: 70 kDa.
IHC-P: 1/50 - 1/100.
ICC/IF: 1/500 - 1/1000.
Target
Relevance
KCNC4 (Potassium voltage gated channel subfamily C member 4) belongs to the delayed rectifier class of channel proteins and is an integral membrane protein that mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient.
Cellular localization
Membrane; Multi-pass membrane protein.
Alternative names
- HKSHIIIC antibody
- KCNC 4 antibody
- KSHIIIC antibody
see all
Database links
- Entrez Gene: 3749 Human
- Entrez Gene: 99738 Mouse
- Omim: 176265 Human
- SwissProt: Q03721 Human
- SwissProt: Q8R1C0 Mouse
- Unigene: 153521 Human
Anti-KCNC4 antibody images:
Western blot - KCNC4 antibody (ab79173)
All lanes : Anti-KCNC4 antibody (ab79173) at 1/500 dilution
Lane 1 : extracts from COS7 cells, treated
with Anisomycin (25 µg/ml, 30 mins)
Lane 2 : extracts from COS7 cells, treated
with Anisomycin (25 µg/ml, 30 mins) with immunizing peptide at 10 µg
Lysates/proteins at 20 µg per lane.
Predicted band size : 70 kDa
Observed band size : 70 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - KCNC4 antibody (ab79173)
Immunohistochemical analysis of KCNC4 in paraffin embedded human brain tissue using a 1/50 dilution of ab79173, with (right) or without (left) pre treatment with the immunizing peptide.
Immunocytochemistry/ Immunofluorescence - KCNC4 antibody (ab79173)
Immunofluorescence analysis of KCNC4 in HeLa cells, using a 1/500 dilution of ab79173 with (right) or without (left) pre treatment with the immunizing peptide.
References for Anti-KCNC4 antibody (ab79173)
ab79173 has not yet been referenced specifically in any publications.
Publishing research using ab79173? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"