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Products:Neuroscience >> Neurotransmission >> Receptors / Channels >> Potassium Channels
Overview
Product name
Anti-KCNE2 antibody
See all KCNE2 products (2) ...
Description
Rabbit polyclonal to KCNE2
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human KCNE2.
(Peptide available as ab86641.)
Positive control
This antibody gave a positive signal in mouse pancreas tissue lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Heart >> Cardiac arrhythmias
Neuroscience >> Neurotransmission >> Receptors / Channels >> Potassium Channels
Applications
Show applications key
Our Abpromise guarantee covers the use of ab69376 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent concentration. Detects a band of approximately 17 kDa (predicted molecular weight: 14 kDa).
IHC-P: Use a concentration of 5 µg/mlPerform heat mediated antigen retrieval before commencing with IHC staining protocol.
Target
Function
Ancillary protein that assembles as a beta subunit with a voltage-gated potassium channel complex of pore-forming alpha subunits. Modulates the gating kinetics and enhances stability of the channel complex. Associated with KCNH2/HERG is proposed to form the rapidly activating component of the delayed rectifying potassium current in heart (IKr). May associate with KCNQ2 and/or KCNQ3 and modulate the native M-type current. May associate with KCNQ1/KVLTQ1 and elicit a voltage-independent current. May associate with HCN1 and HCN2 and increase potassium current.
Tissue specificity
Highly expressed in brain, heart, skeletal muscle, pancreas, placenta, kidney, colon and thymus. A small but significant expression is found in liver, ovary, testis, prostate, small intestine and leukocytes. Very low expression, nearly undetectable, in lung and spleen.
Involvement in disease
Defects in KCNE2 are the cause of long QT syndrome type 6 (LQT6) [MIM:613693]. Long QT syndromes are heart disorders characterized by a prolonged QT interval on the ECG and polymorphic ventricular arrhythmias. They cause syncope and sudden death in response to exercise or emotional stress. KCNE2 mutants form channels that open slowly and close rapidly, thereby diminishing potassium currents.
Defects in KCNE2 are the cause of atrial fibrillation familial type 4 (ATFB4) [MIM:611493]. Atrial fibrillation is a common disorder of cardiac rhythm that is hereditary in a small subgroup of patients. It is characterized by disorganized atrial electrical activity and ineffective atrial contraction promoting blood stasis in the atria and reduces ventricular filling. It can result in palpitations, syncope, thromboembolic stroke, and congestive heart failure.
Sequence similarities
Belongs to the potassium channel KCNE family.
Cellular localization
Membrane.
Target information above from: UniProt accessionQ9Y6J6
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- cardiac voltage gated potassium channel accessory subunit 2 antibody
- Kcne2 antibody
- KCNE2_HUMAN antibody
see all
Database links
- Entrez Gene: 9992 Human
- Entrez Gene: 246133 Mouse
- Omim: 603796 Human
- SwissProt: Q9Y6J6 Human
- SwissProt: Q9D808 Mouse
- Unigene: 551521 Human
- Unigene: 679753 Human
- Unigene: 156736 Mouse
Anti-KCNE2 antibody images:
Western blot - KCNE2 antibody (ab69376)
Anti-KCNE2 antibody (ab69376) at 1 µg/ml + Pancreas (Mouse) Tissue Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Predicted band size : 14 kDa
Observed band size : 17 kDa (why is the actual band size different from the predicted?)
KCNE2 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. Furthermore, this reaction is also observed in other commercially available antibodies to KCNE2.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - KCNE2 antibody (ab69376)
IHC image of KCNE2 staining in human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab69376, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
References for Anti-KCNE2 antibody (ab69376)
ab69376 has not yet been referenced specifically in any publications.
Publishing research using ab69376? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"