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Lane 1 : Marker
Lane 2 : Anti-KCNG1 antibody (ab49063) at 0.25 µg/ml
Lane 1 : As above
Lane 2 : HepG2 cell lysate at 10 µg with 5% skimmed milk in PBS.
Secondary
Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 56 kDa
Observed band size : 58 kDa (why is the actual band size different from the predicted?)
Gel concentration: 12%
0
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