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Western blot - KCNH5 antibody (ab32975)
Western blot - KCNH5 antibody (ab32975)
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Immunohistochemistry (Paraffin-embedded sections) - KCNH5 antibody (ab32975)
Immunohistochemistry (Paraffin-embedded sections) - KCNH5 antibody (ab32975)
(enlarge)


Product Name 

KCNH5 antibody

 

See all KCNH5 antibodies (2)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to KCNH5

Immunogen 

Synthetic peptide derived from a region between amino acids 156-205 of Human KCNH5. Note: The exact sequence is proprietary.

Reacts with 
(species key)

Hu

Tested applications 
(see key)

ELISA, IHC-P, WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
ELISA: 1/312500.
IHC-P: Use at a concentration of 4 - 8 µg/ml.
WB: Use at a concentration of 0.5 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 112 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

HepG2 cell lysate and human brain tissue.

Cellular localization 

Cell Membrane: multi-pass membrane protein.

Research areas 

Signal Transduction >> Metabolism >> Plasma Membrane >> Channels
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calmodulin Pathway
Neuroscience >> Neurotransmission >> Receptors / Channels >> Potassium Channels

Relevance 

Voltage-gated potassium (Kv) channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. The KCNH5 gene encodes a member of the potassium channel, voltage-gated, subfamily H. This member is a pore-forming (alpha) subunit of a voltage-gated non-inactivating delayed rectifier potassium channel. KCNH5 is not expressed in differentiating myoblasts.

 

Alternative names KCNH5 antibody (ab32975)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

27133    (Human)

Omim

    

605716    (Human)

SwissProt

    

Q8NCM2    (Human)

Unigene

    

27043    (Human)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: None
Constituents: 2% Sucrose, PBS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this KCNH5 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for KCNH5 antibody images, references, products related to ab32975 and other tools.

 

KCNH5 antibody images:

Product image  Western blot - KCNH5 antibody (ab32975)

Western blot - KCNH5 antibody (ab32975)

KCNH5 antibody (ab32975) at 0.5 µg/ml + HepG2 cell lysate

Secondary
HRP-conjugated anti-rabbit IgG at 1/50000 dilution

Predicted band size : 112 kDa
Observed band size : 60 kDa (why is the actual band size different from the predicted?)

 

Product image  Immunohistochemistry (Paraffin-embedded sections) - KCNH5 antibody (ab32975)

Immunohistochemistry (Paraffin-embedded sections) - KCNH5 antibody (ab32975)

ab32975, at 4 ug/ml concentration, staining paraffin-embedded human brain tissue by immmunohistochemistry. Neural cells with positive label are indicated by arrows.

 
 

Search PubMed (MEDLINE) for references to KCNH5

 

KCNH5 antibody - more information

Customer reviews (feedback) regarding KCNH5 antibody

Customer FAQs regarding KCNH5 antibody

Protocols for KCNH5 antibody

Price and availability of products related to KCNH5 antibody

 

KCNH5 antibody - related products:

Products for Western blot

Loading controls

Lysates

 

Positive Controls for KCNH5 antibody

ab7900

  

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

 

Compatible Secondaries for KCNH5 antibody

ab6564

  

Cy5 ® antibody

ab6717

  

FITC antibody

ab6720

  

Biotin antibody

ab6721

  

HRP antibody

ab6722

  

Alkaline Phosphatase antibody

ab6793

  

Texas Red ® antibody

ab7007

  

Phycoerythrin antibody

ab7019

  

Agarose antibody

ab27234

  

10nm Gold antibody

ab27235

  

5nm Gold antibody

ab27236

  

15nm Gold antibody

ab27237

  

20nm Gold antibody

ab27238

  

30nm Gold antibody

ab27239

  

10nm Gold antibody

ab27240

  

5nm Gold antibody

ab31193

  

Duck polyclonal to Rabbit IgG

ab60314

  

Chromeo™ 488 antibody

ab60315

  

Chromeo™ 494 antibody

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Chromeo™ 546 antibody

ab60319

  

Chromeo™ 642 antibody

 

Other KCNH5 Antibodies

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KCNH5 antibody - other tools:

Contact Abcam with a Technical Enquiry about KCNH5 antibody

 

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