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Products:Neuroscience >> Neurotransmission >> Receptors / Channels >> Potassium Channels
Overview
Product name
Anti-KCNH5 antibody
See all KCNH5 products (2) ...
Description
Rabbit polyclonal to KCNH5
Tested applications
ELISA, WB, IHC-Pmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Cow, Dog, Zebrafish
Immunogen
Synthetic peptide derived from a region between amino acids 156-205 of Human KCNH5. Note: The exact sequence is proprietary.
Positive control
HepG2 cell lysate and human brain tissue.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Metabolism >> Plasma Membrane >> Channels
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calmodulin Pathway
Neuroscience >> Neurotransmission >> Receptors / Channels >> Potassium Channels
Applications
Show applications key
Our Abpromise guarantee covers the use of ab32975 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/312500ELISA: 1/312500
WB: Use a concentration of 0.5 µg/mlDetects a band of approximately 60 kDa (predicted molecular weight: 112 kDa).
IHC-P: Use a concentration of 4 - 8 µg/ml.
Target
Relevance
Voltage-gated potassium (Kv) channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. The KCNH5 gene encodes a member of the potassium channel, voltage-gated, subfamily H. This member is a pore-forming (alpha) subunit of a voltage-gated non-inactivating delayed rectifier potassium channel. KCNH5 is not expressed in differentiating myoblasts.
Cellular localization
Cell Membrane: multi-pass membrane protein.
Alternative names
- EAG2 antibody
- Ether a go go potassium channel 2 antibody
- H EAG2 antibody
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Database links
- Entrez Gene: 27133 Human
- Entrez Gene: 238271 Mouse
- Entrez Gene: 171146 Rat
- Omim: 605716 Human
- SwissProt: Q8NCM2 Human
- SwissProt: Q6NZH0 Mouse
- SwissProt: Q920E3 Mouse
- SwissProt: Q9EPI9 Rat
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Anti-KCNH5 antibody images:
Western blot - KCNH5 antibody (ab32975)
Anti-KCNH5 antibody (ab32975) at 0.5 µg/ml + HepG2 cell lysate
Secondary
HRP-conjugated anti-rabbit IgG at 1/50000 dilution
Predicted band size : 112 kDa
Observed band size : 60 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Paraffin-embedded sections) - KCNH5 antibody (ab32975)
ab32975, at 4 ug/ml concentration, staining paraffin-embedded human brain tissue by immmunohistochemistry. Neural cells with positive label are indicated by arrows.
References for Anti-KCNH5 antibody (ab32975)
This product has been referenced in:
- Macaulay ECet al. Hypomethylation of functional retrotransposon-derived genes in the human placenta. Mamm Genome 22:722-35 (2011). IHC-P; Human.Read more (PubMed: 21874386) »
See 1 publication for this product
Publishing research using ab32975? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"