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All lanes : Anti-KCNQ1 antibody - Carboxyterminal end (ab77701) at 1 µg/ml
Lane 1 :
Lane 2 : A498 (Human Kidney Carcinoma) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 75 kDa
Observed band size : 74 kDa (why is the actual band size different from the predicted?)
Exposure time : 150 seconds
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