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Predicted band size : 56 kDa
Observed band size : 56,75 kDa (why is the actual band size different from the predicted?)
Additional bands at : 25 kDa. We are unsure as to the identity of these extra bands.
E coli-derived fusion protein (1), 293 cell extract (2), and Hela cell extract (3) as test antigen. Affinity-purified IgY dilution: 1:2000 (or 1:500 for cell extract), Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development.
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