Anti-KDEL Receptor antibody [KR-10] (ab69659)

Western blot - KDEL antibody [KR-10] (ab69659)

All lanes : Anti-KDEL Receptor antibody [KR-10] (ab69659) at 1/250 dilution

Lane 1 : HeLa cell lysate (heat shocked)
Lane 2 : 3T3 cell lysate
Lane 3 : PC-12 cell lysate
Lane 4 : CHO-K1 cell lysate
Lane 5 : 3T3 cell lysate (heat shocked)
Lane 6 : PC-12 cell lysate (heat shocked)

Lysates/proteins at 20 µg per lane.


Predicted band size : 25 kDa
Observed band size : 25 kDa

Immunohistochemistry (PFA perfusion fixed frozen sections) - KDEL Receptor antibody [KR-10] (ab69659)

ab69659 staining KDEL Receptor in Fruitfly epidermal cell tissue by Immunohistochemistry (PFA perfusion fixed frozen sections).

Image from Norum M et al, PLoS One. 2010 May 24;5(5):e10802, Fig 8.

Immunocytochemistry/ Immunofluorescence - KDEL Receptor antibody [KR-10] (ab69659)

ab69659 staining the KDEL receptor in cultured human HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X (0.5%) and blocked with 3% BSA for 1 hour at 24ºC. Samples were incubated with primary antibody (1/100) for 1 hour at 24ºC. An Alexa Fluor^®488-conjugated Chicken anti-mouse polyclonal was used as the secondary antibody.

This image is courtesy of an anonymous Abreview

Flow Cytometry - Anti-KDEL Receptor antibody [KR-10] (ab69659)

Overlay histogram showing HepG2 cells stained with ab69659 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab69659, 1µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.