KDEL Receptor antibody [KR-10] (ab69659)

Product Name 

KDEL Receptor antibody [KR-10]

Product type 

Primary antibodies

Description 

Mouse monoclonal [KR-10] to KDEL Receptor

Immunogen 

Synthetic peptide derived from the sequence of bovine KDEL Receptor, conjugated to KLH

Reacts with 
(species key)

Hu, Ms, Rat, Chk, Cow, Dog, Dm, Hm, Mk, Pig, Rb, Shp, Xl

Tested applications 
(see key)

ICC/IF, IHC-Fr, IHC-P, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ICC/IF: Use at an assay dependent dilution.
IHC-P: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

HeLa cell lysate (heat shocked), 3T3 cell lysate (heat-shocked and unshocked), PC-12 cell lysate (heat shocked and unshocked) and CHO-K1 cell lysate.

Cellular localization 

Endoplasmic reticulum

Research areas 

Signal Transduction >> Protein Trafficking >> ER Proteins
Tags & Cell Markers >> Subcellular Markers >> Organelles >> ER

Relevance 

Retention of resident soluble proteins in the lumen of the endoplasmic reticulum (ER) is achieved in both yeast and animal cells by their continual retrieval from the cis-Golgi, or a pre-Golgi compartment. Sorting of these proteins is dependent on a C-terminal tetrapeptide signal, usually lys-asp-glu-leu (KDEL) in animal cells, and his-asp-glu-leu (HDEL) in S. cerevisiae. This process is mediated by a receptor that recognizes, and binds the tetrapeptide-containing protein, and returns it to the ER. In yeast, the sorting receptor encoded by a single gene, ERD2, which is a seven-transmembrane protein. Unlike yeast, several human homologs of the ERD2 gene, constituting the KDEL receptor gene family, have been described. The protein encoded by this gene was the first member of the family to be identified, and it encodes a protein structurally and functionally similar to the yeast ERD2 gene product.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

KR-10

Isotype 

IgG1

Purity 

Protein G purified

Storage buffer 

Preservative: 0.09% Sodium Azide
Constituents: 50% Glycerol, PBS, pH 7.2

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

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