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Anti-KDEL Receptor antibody [KR-10] (ab69659)

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Overview

Product name

Anti-KDEL Receptor antibody [KR-10]
See all KDEL Receptor products (2) ...

Description

Mouse monoclonal [KR-10] to KDEL Receptor

Tested applications

ICC/IF, IHC-P, IHC-Fr, IP, IHC-FoFr, WB, Flow Cytmore details

Cross reactivity

Reacts with

Mouse, Rat, Sheep, Rabbit, Chicken, Hamster, Cow, Dog, Human, Pig, Xenopus laevis, Fruit fly (Drosophila melanogaster), Monkey

Immunogen

Synthetic peptide derived from the sequence of bovine KDEL Receptor, conjugated to KLH

Positive control

HeLa cell lysate (heat shocked), 3T3 cell lysate (heat-shocked and unshocked), PC-12 cell lysate (heat shocked and unshocked) and CHO-K1 cell lysate.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.09% Sodium Azide
Constituents: 50% Glycerol, PBS, pH 7.2

Concentration

Concentration information loading...

Purity

Protein G purified

Clonality

Monoclonal

Clone number

KR-10

Isotype

IgG1

Research areas

Signal Transduction >> Protein Trafficking >> ER Proteins
Tags & Cell Markers >> Subcellular Markers >> Organelles >> ER

  • Western blot - KDEL antibody [KR-10] (ab69659)Western blot - KDEL antibody [KR-10] (ab69659) image (enlarge)

  • Immunohistochemistry (PFA perfusion fixed frozen sections) - KDEL Receptor antibody [KR-10] (ab69659)Immunohistochemistry (PFA perfusion fixed frozen sections) - KDEL Receptor antibody [KR-10] (ab69659) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence - KDEL Receptor antibody [KR-10] (ab69659)Immunocytochemistry/ Immunofluorescence - KDEL Receptor antibody [KR-10] (ab69659) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab69659 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

Retention of resident soluble proteins in the lumen of the endoplasmic reticulum (ER) is achieved in both yeast and animal cells by their continual retrieval from the cis-Golgi, or a pre-Golgi compartment. Sorting of these proteins is dependent on a C-terminal tetrapeptide signal, usually lys-asp-glu-leu (KDEL) in animal cells, and his-asp-glu-leu (HDEL) in S. cerevisiae. This process is mediated by a receptor that recognizes, and binds the tetrapeptide-containing protein, and returns it to the ER. In yeast, the sorting receptor encoded by a single gene, ERD2, which is a seven-transmembrane protein. Unlike yeast, several human homologs of the ERD2 gene, constituting the KDEL receptor gene family, have been described. The protein encoded by this gene was the first member of the family to be identified, and it encodes a protein structurally and functionally similar to the yeast ERD2 gene product.

Cellular localization

Endoplasmic reticulum

Alternative names

  • ER lumen protein retaining receptor 1 antibody
  • ERD 2 antibody
  • ERD2 antibody
  • ERD2.1 antibody
  • ERD21 antibody
  • HDEL antibody
  • KDEL (Lys Asp Glu Leu) endoplasmic reticulum protein retention receptor 1 antibody
  • KDEL (Lys-Asp-Glu-Leu) 72 kD endoplasmic reticulum protein retention receptor 1 antibody
  • KDEL endoplasmic reticulum protein retention receptor 1 antibody
  • KDEL R1 antibody
  • KDEL receptor 1 antibody
  • KDELR 1 antibody
  • KDELR1 antibody
  • PM 23 antibody
  • PM23 antibody
  • Putative MAPK activating protein PM23 antibody
see all

Database links

see all

Anti-KDEL Receptor antibody [KR-10] images:

  Western blot - KDEL antibody [KR-10] (ab69659)

Western blot - KDEL antibody [KR-10] (ab69659)

All lanes : Anti-KDEL Receptor antibody [KR-10] (ab69659) at 1/250 dilution

Lane 1 : HeLa cell lysate (heat shocked)
Lane 2 : 3T3 cell lysate
Lane 3 : PC-12 cell lysate
Lane 4 : CHO-K1 cell lysate
Lane 5 : 3T3 cell lysate (heat shocked)
Lane 6 : PC-12 cell lysate (heat shocked)

Lysates/proteins at 20 µg per lane.


Predicted band size : 25 kDa
Observed band size : 25 kDa

  Immunohistochemistry (PFA perfusion fixed frozen sections) - KDEL Receptor antibody [KR-10] (ab69659)

Immunohistochemistry (PFA perfusion fixed frozen sections) - KDEL Receptor antibody [KR-10] (ab69659)

ab69659 staining KDEL Receptor in Fruitfly epidermal cell tissue by Immunohistochemistry (PFA perfusion fixed frozen sections).

Image from Norum M et al, PLoS One. 2010 May 24;5(5):e10802, Fig 8.

  Immunocytochemistry/ Immunofluorescence - KDEL Receptor antibody [KR-10] (ab69659)

Immunocytochemistry/ Immunofluorescence - KDEL Receptor antibody [KR-10] (ab69659)

ab69659 staining the KDEL receptor in cultured human HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X (0.5%) and blocked with 3% BSA for 1 hour at 24°C. Samples were incubated with primary antibody (1/100) for 1 hour at 24°C. An Alexa Fluor®488-conjugated Chicken anti-mouse polyclonal was used as the secondary antibody.

This image is courtesy of an anonymous Abreview

See Abreview

  Flow Cytometry - Anti-KDEL Receptor antibody [KR-10] (ab69659)

Flow Cytometry - Anti-KDEL Receptor antibody [KR-10] (ab69659)

Overlay histogram showing HepG2 cells stained with ab69659 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab69659, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-KDEL Receptor antibody [KR-10] (ab69659)

This product has been referenced in:

  • Kim Set al. The novel endosomal membrane protein Ema interacts with the class C Vps-HOPS complex to promote endosomal maturation. J Cell Biol 188:717-34 (2010). Fruit fly (Drosophila melanogaster).Read more (PubMed: 20194640) »
  • Norum Met al. Trafficking through COPII stabilises cell polarity and drives secretion during Drosophila epidermal differentiation. PLoS One 5:e10802 (2010). IHC-FoFr; Fruit fly (Drosophila melanogaster).Read more (PubMed: 20520821) »

See all 2 publications for this product

Publishing research using ab69659? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"