![Western blot - LSD1 antibody [1B2E5] (ab31954) image](#ab31954_WB.jpg)
![Immunohistochemistry (Paraffin-embedded sections) - LSD1 antibody [1B2E5] (ab31954) image](#ab31954_IHC_1.jpg)
![Immunohistochemistry (Paraffin-embedded sections) - LSD1 antibody [1B2E5] (ab31954) image](#ab31954_IHC_2.gif)
Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Methylation >> Lysine demethylation
Overview
Product name
Anti-KDM1 / LSD1 antibody [1B2E5 + 1B2F2]
See all KDM1 / LSD1 products (10) ...
Description
Mouse monoclonal [1B2E5 + 1B2F2] to KDM1 / LSD1
Tested applications
IHC-FoFr, IHC-P, IHC-Fr, ELISA, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Ni-NTA purified truncated recombinant human LSD1 expressed in E. Coli strain BL21 (DE3).
Positive control
HeLa and Jurkat cell lysate for Western blot. Human kidnay and lung carcinoma tissue for Immunohistochemistry.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: Ascites
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Clone number
1B2E5 + 1B2F2
Isotype
IgG1
Research areas
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Methylation >> Lysine demethylation
Applications
Show applications key
Our Abpromise guarantee covers the use of ab31954 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
ELISA: 1/10000.
IHC-Fr: 1/500 - 1/2000.
IHC-FoFr: Use at an assay dependent dilution (PMID 19817750).
IHC-P: 1/500 - 1/2000.
WB: 1/500 - 1/2000. Predicted molecular weight: ~93 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Histone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis.
Tissue specificity
Ubiquitously expressed.
Sequence similarities
Belongs to the flavin monoamine oxidase family.
Contains 1 SWIRM domain.
Domain
The SWIRM domain may act as an anchor site for a histone tail.
Cellular localization
Nucleus.
Target information above from: UniProt accessionO60341
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Amine oxidase flavin containing domain 2 antibody
- Amine oxidase flavin containing domain protein 2 antibody
- AOF 2 antibody
see all
Database links
- Entrez Gene: 23028 Human
- Omim: 609132 Human
- SwissProt: O60341 Human
- Unigene: 591518 Human
Anti-KDM1 / LSD1 antibody [1B2E5 + 1B2F2] images:
Western blot - LSD1 antibody [1B2E5] (ab31954)
![Western blot - LSD1 antibody [1B2E5] (ab31954)](/ps/datasheet/Images/31/ab31954/ab31954_WB.jpg)
All lanes : Anti-KDM1 / LSD1 antibody [1B2E5 + 1B2F2] (ab31954)
Lane 1 : Molecular weight marker
Lane 2 : LSD1 recombinant protein (NB: truncated form)
Lane 3 : HeLa cell lysate
Predicted band size : 93 kDa
Primary antibody dilution range 1:500-1:2000.
Immunohistochemistry (Paraffin-embedded sections) - LSD1 antibody [1B2E5] (ab31954)
![Immunohistochemistry (Paraffin-embedded sections) - LSD1 antibody [1B2E5] (ab31954)](/ps/datasheet/Images/31/ab31954/ab31954_IHC_1.jpg)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, showing nuclear localisation using LSD1 antibody (ab31954) dilution 1:500-1:2000 and an HRP-conjugated secondary antibody with DAB staining.
Immunohistochemistry (Paraffin-embedded sections) - LSD1 antibody [1B2E5] (ab31954)
![Immunohistochemistry (Paraffin-embedded sections) - LSD1 antibody [1B2E5] (ab31954)](/ps/datasheet/Images/31/ab31954/ab31954_IHC_2.gif)
Immunohistochemical analysis of paraffin-embedded human kidney carcinoma tissue, showing nuclear localisation using LSD1 antibody (ab31954) dilution 1:500-1:2000 and an HRP-conjugated secondary antibody with DAB staining.
References for Anti-KDM1 / LSD1 antibody [1B2E5 + 1B2F2] (ab31954)
This product has been referenced in:
- Hirono Set al. Molecular markers associated with lymph node metastasis in pancreatic ductal adenocarcinoma by genome-wide expression profiling. Cancer Sci : (2009). IHC-FoFr; Human.Read more (PubMed: 19817750) »
See 1 publication for this product
Publishing research using ab31954? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"