Anti-KDM1 / LSD1 antibody - ChIP Grade (ab17721)

  Immunocytochemistry/ Immunofluorescence - LSD1 antibody (ab17721)

ICC/IF image of ab17721 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab17721, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iT^TM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

  ChIP - LSD1 antibody - ChIP Grade (ab17721)

     ab17721 at a 1/100 dilution in cross-linking ChIP (using formaldehyde) with mouse liver tissue lysate. The protocol from Nguyen, T. T, et al. (2005) Mol. Cell. Biol. 25: 2147-2157 was followed. Semiquantitative PCR was used as the detection step.

Lane 1: Input

Lane 2: ab17721

Lane 3: IgG

Lane 4: No ab

This image is courtesy of an Abreview by  Michelle Barton.

See Abreview

  Western blot - LSD1 antibody - ChIP Grade (ab17721)

All lanes : Anti-KDM1 / LSD1 antibody - ChIP Grade (ab17721) at 1 µg/ml

Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)
Lane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)

Lysates/proteins at 20 µg per lane.

Secondary
Goat polyclonal to Rabbit IgG H&L (HRP) Pre-Adsorbed at 1/10000 dilution

Performed under reducing conditions.

Predicted band size : 93 kDa

  Immunofluorescence - LSD1 antibody (ab17721)

Human 293T cells fixed in 4% paraformaldehyde were immunostained with ab17721 (1/200) for LSD1 and detected using FITC labelled anti-rabbit (Green). Nuclear DNA is stained blue with DAPI.

Katia Ancelin