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Immunocytochemistry/ Immunofluorescence - LSD1 antibody (ab17721)
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ChIP - LSD1 antibody - ChIP Grade (ab17721)
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Western blot - LSD1 antibody - ChIP Grade (ab17721)
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Product Name
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KDM1 / LSD1 antibody - ChIP Grade
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See all LSD1 antibodies (9)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to KDM1 / LSD1 - ChIP Grade
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 800 to the C-terminus of Human LSD1.
(Peptide available as ab17763.)
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Reacts with
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Hu, Ms, Pig
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Tested applications
(see key)
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ChIP, ICC/IF, IHC-P, IP, WB
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Abreviews
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Application notes
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Recommended dilutions
ChIP: Use at an assay dependent concentration.
ICC/IF: Use at a concentration of 1 µg/ml.
IHC-P: Use at an assay dependent dilution (PMID 18502896).
IP: Use at an assay dependent concentration.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 105 kDa (predicted molecular weight: 93 kDa). Can be blocked with LSD1 peptide (ab17763).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Methylation >> Lysine demethylation
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Relevance
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Gene activation and repression is specifically regulated by histone methylation status at distinct lysine residues. Lysine specific demethylase 1 (KDM1 / LSD1) is a long-sought histone demethylase that specifically demethylates mono and di methyl histone H3 at K4 and K9. Thus KDM1 is a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. KDM1 is part of a histone deacetylase complex that contains HDAC1, HDAC2, AOF2, RCOR1, ZNF261, ZNF198, KIAA0182 and GTF2I.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this KDM1 / LSD1 antibody - ChIP Grade is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for KDM1 / LSD1 antibody - ChIP Grade images, references, products related to ab17721 and other tools.
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KDM1 / LSD1 antibody - ChIP Grade images:
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 Immunocytochemistry/ Immunofluorescence - LSD1 antibody (ab17721)
ICC/IF image of ab17721 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab17721, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
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ChIP - LSD1 antibody - ChIP Grade (ab17721)
ab17721 at a 1/100 dilution in cross-linking ChIP (using formaldehyde) with mouse liver tissue lysate. The protocol from Nguyen, T. T, et al. (2005) Mol. Cell. Biol. 25: 2147-2157 was followed. Semiquantitative PCR was used as the detection step. Lane 1: Input Lane 2: ab17721 Lane 3: IgG Lane 4: No ab This image is courtesy of an Abreview by Michelle Barton.
See Abreview
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Immunofluorescence - LSD1 antibody (ab17721)
Human 293T cells fixed in 4% paraformaldehyde were immunostained with ab17721 (1/200) for LSD1 and detected using FITC labelled anti-rabbit (Green). Nuclear DNA is stained blue with DAPI. Katia Ancelin
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References for KDM1 / LSD1 antibody - ChIP Grade (ab17721)
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This product has been used in: (two most recent references)
Lee SW et al. ASXL1 represses retinoic acid receptor-mediated transcription through associating with HP1 and LSD1. J Biol Chem 285:18-29 (2010). WB; Human. PubMed: 19880879
Laurent B et al. Gfi-1B promoter remains associated with active chromatin marks throughout erythroid differentiation of human primary progenitor cells. Stem Cells 27:2153-62 (2009). WB, ChIP; Human. PubMed: 19522008
See all 15 publication references for this product.
If you publish research using ab17721 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to KDM1 / LSD1
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KDM1 / LSD1 antibody - ChIP Grade - more information
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