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Products:Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Methylation >> Lysine demethylation
Overview
Product name
Anti-KDM1 / LSD1 antibody
See all KDM1 / LSD1 products (10) ...
Description
Rabbit polyclonal to KDM1 / LSD1
Tested applications
IP, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide, corresponding to a 17 amino acid peptide from near the N terminus of Human LSD1
Positive control
Mouse P815 cell lysate
Properties
Form
Liquid
Storage instructions
Store at +4°C. Do not freeze.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Methylation >> Lysine demethylation
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Western blot - LSD1 antibody (ab37165)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab37165 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at an assay dependent dilution. (PubMed: 20404188)
WB: Use a concentration of 1 - 2 µg/ml.Detects a band of approximately 105 kDa (predicted molecular weight: 93 kDa).
Target
Function
Histone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis.
Tissue specificity
Ubiquitously expressed.
Sequence similarities
Belongs to the flavin monoamine oxidase family.
Contains 1 SWIRM domain.
Domain
The SWIRM domain may act as an anchor site for a histone tail.
Cellular localization
Nucleus.
Target information above from: UniProt accessionO60341
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Amine oxidase flavin containing domain 2 antibody
- Amine oxidase flavin containing domain protein 2 antibody
- AOF 2 antibody
see all
Database links
- Entrez Gene: 23028 Human
- Entrez Gene: 99982 Mouse
- Entrez Gene: 500569 Rat
- Omim: 609132 Human
- SwissProt: O60341 Human
- SwissProt: Q6ZQ88 Mouse
- Unigene: 591518 Human
- Unigene: 28540 Mouse
Anti-KDM1 / LSD1 antibody images:
Western blot - LSD1 antibody (ab37165)
Lane 1 : Anti-KDM1 / LSD1 antibody (ab37165) at 1 µg/ml
Lane 2 : Anti-KDM1 / LSD1 antibody (ab37165) at 2 µg/ml
Lane 1 : Mouse P815 cell lysate
Lane 2 : Mouse P815 cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size : 93 kDa
Observed band size : 105 kDa (why is the actual band size different from the predicted?)
References for Anti-KDM1 / LSD1 antibody (ab37165)
This product has been referenced in:
- Dovey OMet al. Histone deacetylase 1 (HDAC1), but not HDAC2, controls embryonic stem cell differentiation. Proc Natl Acad Sci U S A 107:8242-7 (2010). WB, IP; Mouse.Read more (PubMed: 20404188) »
- Foster CTet al. Lysine-specific demethylase 1 regulates the embryonic transcriptome and CoREST stability. Mol Cell Biol 30:4851-63 (2010). WB; Mouse.Read more (PubMed: 20713442) »
See all 2 publications for this product
Publishing research using ab37165? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"