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Overview
Product name
Anti-KDM3A / JHDM2A antibody - Aminoterminal end
See all KDM3A / JHDM2A products (6) ...
Description
Rabbit polyclonal to KDM3A / JHDM2A - Aminoterminal end
Tested applications
ICC/IF, ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
A KLH conjugated synthetic peptide selected from the N terminal region of human KDM3A / JHDM2A.
Positive control
Human hepatocarcinoma tissue
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Ammonium Sulphate Precipitation
Purification notes
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab75620 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 5 µg/ml
ELISA
ELISA: 1/1000ELISA: 1/1000
IHC-P: 1/10 - 1/50.Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
Target
Function
Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. Involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation. Involved in spermatogenesis by regulating expression of target genes such as PRM1 and TMP1 which are required for packaging and condensation of sperm chromatin. Involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1.
Sequence similarities
Belongs to the JHDM2 histone demethylase family.
Contains 1 JmjC domain.
Domain
The JmjC domain and the C6-type zinc-finger are required for the demethylation activity.
Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs are known to mediate the association with nuclear receptors.
Cellular localization
Cytoplasm. Nucleus. Nuclear in round spermatids. When spermatids start to elongate, localizes to the cytoplasm where it forms distinct foci which disappear in mature spermatozoa.
Target information above from: UniProt accessionQ9Y4C1
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DKFZp686A24246 antibody
- DKFZp686P07111 antibody
- JHDM2A antibody
see all
Database links
- Entrez Gene: 55818 Human
- Omim: 611512 Human
- SwissProt: Q9Y4C1 Human
- Unigene: 557425 Human
Anti-KDM3A / JHDM2A antibody - Aminoterminal end images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - KDM3A / JHDM2A antibody - Aminoterminal end (ab75620)
ab75620, at a dilution of 1/50, staining KDM3A / JHDM2A in formalin fixed and paraffin embedded human hepatocarcinoma tissue by Immunohistochemistry. A peroxidase conjugated secondary antibody was then used, followed by DAB staining.
Immunocytochemistry/ Immunofluorescence - Anti-KDM3A / JHDM2A antibody - Aminoterminal end (ab75620)
ICC/IF image of ab75620 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab75620, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-KDM3A / JHDM2A antibody - Aminoterminal end (ab75620)
This product has been referenced in:
- Du ZMet al. Upregulation of MiR-155 in Nasopharyngeal Carcinoma is Partly Driven by LMP1 and LMP2A and Downregulates a Negative Prognostic Marker JMJD1A. PLoS One 6:e19137 (2011). IHC-P; Human.Read more (PubMed: 21541331) »
See 1 publication for this product
Publishing research using ab75620? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"