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Overview
Product name
Anti-KDM3A / JHDM2A antibody
See all KDM3A / JHDM2A products (6) ...
Description
Rabbit polyclonal to KDM3A / JHDM2A
Tested applications
WB, IPmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide, corresponding to a region within amino acids 1271-1321 of human KDM3A / JHDM2A
Positive control
HeLa whole cell lysate
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7 - 8
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Affinity purified using an epitope specific to KDM3A / JHDM2A immobilized on solid support.
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab80598 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
IP
Application notes
IP: Use at 2-5 µg/mg of lysate.
WB: 1/2000 - 1/10000. Predicted molecular weight: 147 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. Involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation. Involved in spermatogenesis by regulating expression of target genes such as PRM1 and TMP1 which are required for packaging and condensation of sperm chromatin. Involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1.
Sequence similarities
Belongs to the JHDM2 histone demethylase family.
Contains 1 JmjC domain.
Domain
The JmjC domain and the C6-type zinc-finger are required for the demethylation activity.
Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs are known to mediate the association with nuclear receptors.
Cellular localization
Cytoplasm. Nucleus. Nuclear in round spermatids. When spermatids start to elongate, localizes to the cytoplasm where it forms distinct foci which disappear in mature spermatozoa.
Target information above from: UniProt accessionQ9Y4C1
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DKFZp686A24246 antibody
- DKFZp686P07111 antibody
- JHDM2A antibody
see all
Database links
- Entrez Gene: 55818 Human
- Omim: 611512 Human
- SwissProt: Q9Y4C1 Human
- Unigene: 557425 Human
Anti-KDM3A / JHDM2A antibody images:
Western blot - KDM3A / JHDM2A antibody (ab80598)
All lanes : Anti-KDM3A / JHDM2A antibody (ab80598) at 0.1 µg/ml
Lane 1 : whole HeLa cell lysate at 50 µg
Lane 2 : whole HeLa cell lysate at 15 µg
Lane 3 : whole HeLa cell lysate at 5 µg
Predicted band size : 147 kDa
Observed band size : 171 kDa (why is the actual band size different from the predicted?)
Additional bands at : 300 kDa,85 kDa. We are unsure as to the identity of these extra bands.
Immunoprecipitation - KDM3A / JHDM2A antibody (ab80598)
Detection of Human KDM3A / JHDM2A by Immunoprecipitation. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Lane 1: ab80598 used for IP at 3 µg/mg lysate; Lane 2: a control IgG was used for IP. For subsequent Western blot detection, ab80598 was used at 1 µg/ml.
References for Anti-KDM3A / JHDM2A antibody (ab80598)
ab80598 has not yet been referenced specifically in any publications.
Publishing research using ab80598? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"