|
|
|
|
Western blot - KDM3A / JHDM2A antibody (ab80598)
(enlarge)
|
|
Immunoprecipitation - KDM3A / JHDM2A antibody (ab80598)
(enlarge)
|
|
|
|
|
Product Name
|
KDM3A / JHDM2A antibody
|
| |
See all KDM3A / JHDM2A antibodies (4)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to KDM3A / JHDM2A
|
|
Immunogen
|
Synthetic peptide, corresponding to a region within amino acids 1271-1321 of human KDM3A / JHDM2A
|
|
Reacts with
(species key)
|
Hu
|
|
Tested applications
(see key)
|
IP, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
IP: Use at 2-5 µg/mg of lysate.
WB: 1/2000 - 1/10000. Predicted molecular weight: 147 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
HeLa whole cell lysate
|
|
Cellular localization
|
Nuclear
|
|
Research areas
|
Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Jumonji containing proteins
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
|
|
Relevance
|
KDM3A is responsible for the demethylation of mono and di methyl histone H3 K9.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Immunogen affinity purified
|
|
Storage buffer
|
Preservative: 0.09% Sodium Azide
Constituents: PBS, 8mM Phosphate, 60mM Citrate, 150mM Tris, pH 7 - 8 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
|
|
Purification notes
|
Affinity purified using an epitope specific to KDM3A / JHDM2A immobilized on solid support.
|
|
Form
|
Liquid
|
|
Concentration
|
1.000 mg/ml
|
|
Storage instructions
|
Store at +4°C.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this KDM3A / JHDM2A antibody is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for KDM3A / JHDM2A antibody images, references, products related to ab80598 and other tools.
|
| |
KDM3A / JHDM2A antibody images:
|
 Western blot - KDM3A / JHDM2A antibody (ab80598)
All lanes : KDM3A / JHDM2A antibody (ab80598) at 0.1 µg/ml
Lane 1 : whole HeLa cell lysate at 50 µg
Lane 2 : whole HeLa cell lysate at 15 µg
Lane 3 : whole HeLa cell lysate at 5 µg
Predicted band size : 147 kDa
Observed band size : 171 kDa (why is the actual band size different from the predicted?)
Additional bands at : 300 kDa,85 kDa. We are unsure as to the identity of these extra bands.
|
| |
Immunoprecipitation - KDM3A / JHDM2A antibody (ab80598)
Detection of Human KDM3A / JHDM2A by Immunoprecipitation. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Lane 1: ab80598 used for IP at 3 µg/mg lysate; Lane 2: a control IgG was used for IP. For subsequent Western blot detection, ab80598 was used at 1 µg/ml.
|
| |
| |
|
Search PubMed (MEDLINE) for references to KDM3A / JHDM2A
|
| |
KDM3A / JHDM2A antibody - more information
|
Customer reviews (feedback) regarding KDM3A / JHDM2A antibody |
Customer FAQs regarding KDM3A / JHDM2A antibody |
Protocols for KDM3A / JHDM2A antibody |
Price and availability of products related to KDM3A / JHDM2A antibody |
| |
KDM3A / JHDM2A antibody - related products:
|
|
|
| |
KDM3A / JHDM2A antibody - other tools:
|
Contact Abcam with a Technical Enquiry about KDM3A / JHDM2A antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
