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Western blot - KDM3B / JMJD1B antibody (ab66654)
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Product Name
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KDM3B / JMJD1B antibody
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See all KDM3B / JMJD1B antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to KDM3B / JMJD1B
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Immunogen
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Synthetic peptide: NLYSCIKVAE DFVSPEHVKH CFRLTQEFRH LSNTHTNHED KLQVKNIIYH, corresponding to a region within C terminal amino acids 1693-1742 of Human KDM3B / JMJD1B
BLAST 'NLYSCIKVAE DFVSPEHVKH CFRLTQEFRH LSNTHTNHED KLQVKNIIYH ' with  or 
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Reacts with
(species key)
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Hu, Ms, Rat, Dog
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at an assay dependent dilution.
ELISA titre: using peptide based assay: 1:12500
WB: Use at a concentration of 2.5 µg/ml. Predicted molecular weight: 192 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Fetal thymus lysate
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Methylation >> Lysine demethylation
Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Jumonji containing proteins
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
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Relevance
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KDM3B / JHDM2B / JMJD1B is a histone demethylase responsible for demethylating mono methyl histone H3 at K9.
JMJD1B contains a highly-conserved C-terminus containing a zinc finger with the unique spacing Cys-X2-Cys-X7-His-X2-Cys-X2-Cys-X4-Cys-X2-Cys and a jmjC domain. It plays an important role in histone demethylation and may be a candidate for tumor suppressors.
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KDM3B / JMJD1B antibody (ab66654) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein A purified
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Storage buffer
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Preservative: None
Constituents: 2% Sucrose, PBS
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Form
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Lyophilised
100 µl of distilled water.
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this KDM3B / JMJD1B antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for KDM3B / JMJD1B antibody images, references, products related to ab66654 and other tools.
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KDM3B / JMJD1B antibody images:
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 Western blot - KDM3B / JMJD1B antibody (ab66654)
KDM3B / JMJD1B antibody (ab66654) at 2.5 µg/ml + Fetal thymus lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 192 kDa
Observed band size : 192 kDa
Gel concentration: 8%
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Search PubMed (MEDLINE) for references to KDM3B / JMJD1B
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KDM3B / JMJD1B antibody - more information
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Customer reviews (feedback) regarding KDM3B / JMJD1B antibody |
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KDM3B / JMJD1B antibody - related products:
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KDM3B / JMJD1B antibody - other tools:
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Contact Abcam with a Technical Enquiry about KDM3B / JMJD1B antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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