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Overview
Product name
Anti-KDM3B / JMJD1B antibody
See all KDM3B / JMJD1B products (5) ...
Description
Rabbit polyclonal to KDM3B / JMJD1B
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Cow, Dog, Fruit fly (Drosophila melanogaster)
Immunogen
Synthetic peptide: NLYSCIKVAE DFVSPEHVKH CFRLTQEFRH LSNTHTNHED KLQVKNIIYH, corresponding to a region within C terminal amino acids 1693-1742 of Human KDM3B / JMJD1B
NLYSCIKVAE DFVSPEHVKH CFRLTQEFRH LSNTHTNHED KLQVKNIIYH
Positive control
Fetal thymus lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Methylation >> Lysine demethylation
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Jumonji containing proteins
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Methylation
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Western blot - KDM3B / JMJD1B antibody (ab66654)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab66654 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 2.5 µg/mlPredicted molecular weight: 192 kDa.
Target
Function
Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Demethylation of Lys residue generates formaldehyde and succinate. May have tumor suppressor activity.
Tissue specificity
Ubiquitous. Highly expressed in placenta, skeletal muscle, kidney, heart and liver.
Sequence similarities
Belongs to the JHDM2 histone demethylase family.
Contains 1 JmjC domain.
Domain
Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs are known to mediate the association with nuclear receptors.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ7LBC6
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 5qNCA antibody
- C5orf7 antibody
- JHDM2B antibody
see all
Database links
- Entrez Gene: 51780 Human
- Omim: 609373 Human
- SwissProt: Q7LBC6 Human
- SwissProt: Q9BVH6 Human
- SwissProt: Q3TQV8 Mouse
- SwissProt: Q3UI14 Mouse
- SwissProt: Q6ZPY7 Mouse
- Unigene: 483486 Human
see all
Anti-KDM3B / JMJD1B antibody images:
Western blot - KDM3B / JMJD1B antibody (ab66654)
Anti-KDM3B / JMJD1B antibody (ab66654) at 2.5 µg/ml + Fetal thymus lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 192 kDa
Observed band size : 192 kDa
Gel concentration: 8%
References for Anti-KDM3B / JMJD1B antibody (ab66654)
ab66654 has not yet been referenced specifically in any publications.
Publishing research using ab66654? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"