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Western blot - KDM3B / JMJD1B antibody (ab70797)
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Immunoprecipitation - KDM3B / JMJD1B antibody (ab70797)
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Product Name
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KDM3B / JMJD1B antibody
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See all KDM3B / JMJD1B antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to KDM3B / JMJD1B
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Immunogen
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Synthetic peptide corresponding to a region between residue 1710 and the C-terminus (residue 1761) of human KDM3B / JMJD1B (NP_057688.2).
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IP: Use at 2-5µg/mg of lysate.
WB: 1/2000 - 1/10000. Detects a band of approximately 240 kDa (predicted molecular weight: 191 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HeLa and 293T whole cell lysates.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Methylation >> Lysine demethylation
Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Jumonji containing proteins
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
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Relevance
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KDM3B / JHDM2B / JMJD1B is a histone demethylase responsible for demethylating mono methyl histone H3 at K9.
JMJD1B contains a highly-conserved C-terminus containing a zinc finger with the unique spacing Cys-X2-Cys-X7-His-X2-Cys-X2-Cys-X4-Cys-X2-Cys and a jmjC domain. It plays an important role in histone demethylation and may be a candidate for tumor suppressors.
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KDM3B / JMJD1B antibody (ab70797) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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ab70797 was affinity purified using an epitope specific to KDM3B / JMJD1B immobilized on solid support.
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Form
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Liquid
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Concentration
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0.200 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this KDM3B / JMJD1B antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for KDM3B / JMJD1B antibody images, references, products related to ab70797 and other tools.
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KDM3B / JMJD1B antibody images:
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 Western blot - KDM3B / JMJD1B antibody (ab70797)
All lanes : KDM3B / JMJD1B antibody (ab70797) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
developed using the ECL technique
Predicted band size : 191 kDa
Observed band size : 240 kDa (why is the actual band size different from the predicted?)
Additional bands at : 110 kDa,30 kDa,60 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 3 minutes
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Immunoprecipitation - KDM3B / JMJD1B antibody (ab70797)
Detection of KDM3B / JMJD1B by Western Blot of Immunoprecipitate.
ab70797 at 1µg/ml staining KDM3B / JMJD1B in HeLa whole cell lysate immunoprecipitated using ab70797 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.
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Search PubMed (MEDLINE) for references to KDM3B / JMJD1B
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KDM3B / JMJD1B antibody - more information
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Customer reviews (feedback) regarding KDM3B / JMJD1B antibody |
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KDM3B / JMJD1B antibody - related products:
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KDM3B / JMJD1B antibody - other tools:
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