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Overview
Product name
Anti-KDM3B / JMJD1B antibody
See all KDM3B / JMJD1B products (5) ...
Description
Rabbit polyclonal to KDM3B / JMJD1B
Tested applications
WB, IPmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide corresponding to a region between residue 1710 and the C-terminus (residue 1761) of human KDM3B / JMJD1B (NP_057688.2).
Positive control
HeLa and 293T whole cell lysates.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab70797 was affinity purified using an epitope specific to KDM3B / JMJD1B immobilized on solid support.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Methylation >> Lysine demethylation
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Jumonji containing proteins
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Methylation
Applications
Show applications key
Our Abpromise guarantee covers the use of ab70797 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
IP: Use at 2-5µg/mg of lysate.
WB: 1/2000 - 1/10000. Detects a band of approximately 240 kDa (predicted molecular weight: 191 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Demethylation of Lys residue generates formaldehyde and succinate. May have tumor suppressor activity.
Tissue specificity
Ubiquitous. Highly expressed in placenta, skeletal muscle, kidney, heart and liver.
Sequence similarities
Belongs to the JHDM2 histone demethylase family.
Contains 1 JmjC domain.
Domain
Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs are known to mediate the association with nuclear receptors.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ7LBC6
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 5qNCA antibody
- C5orf7 antibody
- JHDM2B antibody
see all
Database links
- Entrez Gene: 51780 Human
- Omim: 609373 Human
- SwissProt: Q7LBC6 Human
- SwissProt: Q9BVH6 Human
- Unigene: 483486 Human
Anti-KDM3B / JMJD1B antibody images:
Western blot - KDM3B / JMJD1B antibody (ab70797)
All lanes : Anti-KDM3B / JMJD1B antibody (ab70797) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
developed using the ECL technique
Predicted band size : 191 kDa
Observed band size : 240 kDa (why is the actual band size different from the predicted?)
Additional bands at : 110 kDa,30 kDa,60 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 3 minutes
Immunoprecipitation - KDM3B / JMJD1B antibody (ab70797)
Detection of KDM3B / JMJD1B by Western Blot of Immunoprecipitate.
ab70797 at 1µg/ml staining KDM3B / JMJD1B in HeLa whole cell lysate immunoprecipitated using ab70797 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.
References for Anti-KDM3B / JMJD1B antibody (ab70797)
ab70797 has not yet been referenced specifically in any publications.
Publishing research using ab70797? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"