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Western blot - JHDM3A / JMJD2A antibody [3559D5a] (ab54388)
Western blot - JHDM3A / JMJD2A antibody [3559D5a] (ab54388)
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Product Name 

KDM4A / JHDM3A / JMJD2A antibody [3559D5a]

 

See all KDM4A / JHDM3A / JMJD2A antibodies (5)...

Product type 

Primary antibodies

Description 

Mouse monoclonal [3559D5a] to KDM4A / JHDM3A / JMJD2A

Immunogen 

Recombinant fragment

Reacts with 
(species key)

Hu

Tested applications 
(see key)

Dot, WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
Dot: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Detects a band of approximately 60 kDa (predicted molecular weight: 121 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Nuclear

Research areas 

Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Methylation >> Lysine demethylation
Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Jumonji containing proteins
Chromatin and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors

Relevance 

The JHDM3A/JMJD2A gene is a member of the Jumonji domain 2 (JMJD2) family and encodes a protein with a JmjC domain, a JmjN domain, a JD2H domain, two TUDOR domains, and two PHD-type zinc fingers. KDM4A specifically demethylates histone H3 at K9 and K36.

 

Alternative names KDM4A / JHDM3A / JMJD2A antibody [3559D5a] (ab54388)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

9682    (Human)

Omim

    

609764    (Human)

SwissProt

    

O75164    (Human)

Unigene

    

155983    (Human)

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

3559D5a

Isotype 

IgG2a

Purity 

Protein G purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.100 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Notes 

ab54388 was filtered through a 0.22 µm membrane.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this KDM4A / JHDM3A / JMJD2A antibody [3559D5a] is on this datasheet. But please do contact us if you would like any reassurance!


See below for KDM4A / JHDM3A / JMJD2A antibody [3559D5a] images, references, products related to ab54388 and other tools.

 

KDM4A / JHDM3A / JMJD2A antibody [3559D5a] images:

Product image  Western blot - JHDM3A / JMJD2A antibody [3559D5a] (ab54388)

Western blot - JHDM3A / JMJD2A antibody [3559D5a] (ab54388)



Predicted band size : 121 kDa
Observed band size : 60 kDa (why is the actual band size different from the predicted?)


Western blot analysis of immunised recombinant protein fragment, using ab54388

 
 

Search PubMed (MEDLINE) for references to KDM4A / JHDM3A / JMJD2A

 

KDM4A / JHDM3A / JMJD2A antibody [3559D5a] - more information

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Protocols for KDM4A / JHDM3A / JMJD2A antibody [3559D5a]

Price and availability of products related to KDM4A / JHDM3A / JMJD2A antibody [3559D5a]

 

KDM4A / JHDM3A / JMJD2A antibody [3559D5a] - related products:

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Loading controls

Lysates

 

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KDM4A / JHDM3A / JMJD2A antibody [3559D5a] - other tools:

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