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Anti-KDM4A / JHDM3A / JMJD2A antibody [3559D5a] (ab54388)

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Overview

Product name

Anti-KDM4A / JHDM3A / JMJD2A antibody [3559D5a]
See all KDM4A / JHDM3A / JMJD2A products (8) ...

Description

Mouse monoclonal [3559D5a] to KDM4A / JHDM3A / JMJD2A

Tested applications

WB, Dot Blotmore details

Cross reactivity

Reacts with

Recombinant Fragment

Predicted to work with

Human

Immunogen

Recombinant fragment

General notes

ab54388 was filtered through a 0.22 µm membrane.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.05% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4

Concentration

Concentration information loading...

Purity

Protein G purified

Clonality

Monoclonal

Clone number

3559D5a

Isotype

IgG2a

Research areas

Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Methylation >> Lysine demethylation
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Jumonji containing proteins
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors

  • Western blot - JHDM3A / JMJD2A antibody [3559D5a] (ab54388)Western blot - JHDM3A / JMJD2A antibody [3559D5a] (ab54388) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab54388 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: Use at an assay depe...Read more →

    WB: Use at an assay dependent dilution. Detects a band of approximately 60 kDa (predicted molecular weight: 121 kDa).

  • ShowHide

    Dot Blot

     Dot: Use at an assay dep...Read more →

    Dot: Use at an assay dependent dilution.

Target

Function

Histone demethylase that specifically demethylates 'Lys-9' and 'Lys-36' residues of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27' nor H4 'Lys-20'. Demethylates trimethylated H3 'Lys-9' and H3 'Lys-36' residue, while it has no activity on mono- and dimethylated residues. Demethylation of Lys residue generates formaldehyde and succinate. Participates in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1, respectively.

Tissue specificity

Ubiquitous.

Sequence similarities

Belongs to the JHDM3 histone demethylase family.
Contains 1 JmjC domain.
Contains 1 JmjN domain.
Contains 2 PHD-type zinc fingers.
Contains 2 Tudor domains.

Domain

The 2 Tudor domains recognize and bind methylated histone H3 'Lys-4' residue. Double Tudor domain has an interdigitated structure and the unusual fold is required for its ability to bind methylated histone tails. Trimethylated H3 'Lys-4' is bound in a cage of 3 aromatic residues, 2 of which are from the Tudor domain 2, while the binding specificity is determined by side-chain interactions involving residues from the Tudor domain 1. The Tudor domains are able to bind trimethylated histone H3 'Lys-4', trimethylated histone H3 'Lys-9', di- and trimethylated H4 'Lys-20'.

Cellular localization

Nucleus.

Target information above from: UniProt accessionO75164 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • JmjC domain containing histone demethylation protein 3A antibody
  • JmjC domain-containing histone demethylation protein 3A antibody
  • JMJD2 antibody
  • jumonji C domain containing histone demethylase 3A antibody
  • Jumonji domain containing 2 antibody
  • Jumonji domain containing 2A antibody
  • Jumonji domain containing protein 2A antibody
  • Jumonji domain-containing protein 2A antibody
  • KDM4A antibody
  • KDM4A_HUMAN antibody
  • KIAA0677 antibody
  • Lysine-specific demethylase 4A antibody
see all

Database links

Anti-KDM4A / JHDM3A / JMJD2A antibody [3559D5a] images:

  Western blot - JHDM3A / JMJD2A antibody [3559D5a] (ab54388)

Western blot - JHDM3A / JMJD2A antibody [3559D5a] (ab54388)



Predicted band size : 121 kDa
Observed band size : 60 kDa (why is the actual band size different from the predicted?)


Western blot analysis of immunised recombinant protein fragment, using ab54388

References for Anti-KDM4A / JHDM3A / JMJD2A antibody [3559D5a] (ab54388)

ab54388 has not yet been referenced specifically in any publications.

Publishing research using ab54388? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"