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Products:Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors
Overview
Product name
Anti-KDM4A / JHDM3A / JMJD2A antibody - ChIP Grade
See all KDM4A / JHDM3A / JMJD2A products (8) ...
Description
Rabbit polyclonal to KDM4A / JHDM3A / JMJD2A - ChIP Grade
Tested applications
IP, ChIP, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Rat
Immunogen
Recombinant fragment, corresponding to amino acids 592-720 of Human JMJD2A
Positive control
HeLa cell nuclear lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Methylation >> Lysine demethylation
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Jumonji containing proteins
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors
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Western blot - JMJD2A antibody - ChIP Grade (ab24545)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab24545 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at an assay dependent dilution.
ChIP: Use at an assay dependent dilution.
WB: 1/2000Detects a band of approximately 130 kDa (predicted molecular weight: 120 kDa).
Target
Function
Histone demethylase that specifically demethylates 'Lys-9' and 'Lys-36' residues of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27' nor H4 'Lys-20'. Demethylates trimethylated H3 'Lys-9' and H3 'Lys-36' residue, while it has no activity on mono- and dimethylated residues. Demethylation of Lys residue generates formaldehyde and succinate. Participates in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1, respectively.
Tissue specificity
Ubiquitous.
Sequence similarities
Belongs to the JHDM3 histone demethylase family.
Contains 1 JmjC domain.
Contains 1 JmjN domain.
Contains 2 PHD-type zinc fingers.
Contains 2 Tudor domains.
Domain
The 2 Tudor domains recognize and bind methylated histone H3 'Lys-4' residue. Double Tudor domain has an interdigitated structure and the unusual fold is required for its ability to bind methylated histone tails. Trimethylated H3 'Lys-4' is bound in a cage of 3 aromatic residues, 2 of which are from the Tudor domain 2, while the binding specificity is determined by side-chain interactions involving residues from the Tudor domain 1. The Tudor domains are able to bind trimethylated histone H3 'Lys-4', trimethylated histone H3 'Lys-9', di- and trimethylated H4 'Lys-20'.
Cellular localization
Nucleus.
Target information above from: UniProt accessionO75164
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- JMJ2A antibody
- JmjC domain containing histone demethylation protein 3A antibody
- JmjC domain-containing histone demethylation protein 3A antibody
see all
Database links
- Entrez Gene: 9682 Human
- Entrez Gene: 313539 Rat
- Omim: 609764 Human
- SwissProt: O75164 Human
- Unigene: 155983 Human
- Unigene: 95937 Rat
Anti-KDM4A / JHDM3A / JMJD2A antibody - ChIP Grade images:
Western blot - JMJD2A antibody - ChIP Grade (ab24545)
Anti-KDM4A / JHDM3A / JMJD2A antibody - ChIP Grade (ab24545) at 1/2000 dilution + HeLa nuclear extract
Predicted band size : 120 kDa
Observed band size : 130 kDa (why is the actual band size different from the predicted?)
References for Anti-KDM4A / JHDM3A / JMJD2A antibody - ChIP Grade (ab24545)
This product has been referenced in:
- Klose RJet al. The transcriptional repressor JHDM3A demethylates trimethyl histone H3 lysine 9 and lysine 36. Nature : (2006).Read more (PubMed: 16732292) »
- Chang PCet al. Histone Demethylase JMJD2A Regulates Kaposi's Sarcoma-Associated Herpesvirus Replication and Is Targeted by a Viral Transcriptional Factor. J Virol 85:3283-93 (2011).Read more (PubMed: 21228229) »
See all 6 publications for this product
Publishing research using ab24545? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"