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Products:Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors
Anti-KDM4A / JHDM3A / JMJD2A antibody (ab70786)
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Secondary antibodies
- Lysates
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - KDM4A / JHDM3A / JMJD2A antibody (ab70786)
All lanes : Anti-KDM4A / JHDM3A / JMJD2A antibody (ab70786) at 0.04 µg/ml
Lane 1 : Nuclear extract from 293T cells at 50 µg
Lane 2 : Nuclear extract from 293T cells at 15 µg
Lane 3 : Nuclear extract from 293T cells at 5 µg
Lane 4 : Whole cell lysate from HeLa cells at 50 µg
Lane 5 : Whole cell lysate from NIH3T3 cells at 50 µg
Predicted band size : 121 kDa
Observed band size : 130 kDa (why is the actual band size different from the predicted?)
Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.
Immunoprecipitation - KDM4A / JHDM3A / JMJD2A antibody (ab70786)
Detection of Human KDM4A / JHDM3A / JMJD2A by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab70786 at 3 µg/mg lysate (Lane 1). Lane 2 represents IgG IP control. Subsequent Western blot detection of KDM4A / JHDM3A / JMJD2A was performed using ab70786 at 1 µg/ml.
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