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Products:Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors
Overview
Product name
Anti-KDM4A / JHDM3A / JMJD2A antibody
See all KDM4A / JHDM3A / JMJD2A products (8) ...
Description
Rabbit polyclonal to KDM4A / JHDM3A / JMJD2A
Tested applications
WB, IPmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide corresponding to a region between residue 1010 and the C-terminus (residue 1064) of human KDM4A / JHDM3A / JMJD2A. (NP_055478.1)
Positive control
Nuclear extract from 293T cells. Whole cell lysates from HeLa and NIH3T3 cells.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Methylation >> Lysine demethylation
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Jumonji containing proteins
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors
Applications
Show applications key
Our Abpromise guarantee covers the use of ab70786 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
IP: Use at 2-5µg/mg of lysate.
WB: 1/2000 - 1/10000. Detects a band of approximately 130 kDa (predicted molecular weight: 121 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Histone demethylase that specifically demethylates 'Lys-9' and 'Lys-36' residues of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27' nor H4 'Lys-20'. Demethylates trimethylated H3 'Lys-9' and H3 'Lys-36' residue, while it has no activity on mono- and dimethylated residues. Demethylation of Lys residue generates formaldehyde and succinate. Participates in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1, respectively.
Tissue specificity
Ubiquitous.
Sequence similarities
Belongs to the JHDM3 histone demethylase family.
Contains 1 JmjC domain.
Contains 1 JmjN domain.
Contains 2 PHD-type zinc fingers.
Contains 2 Tudor domains.
Domain
The 2 Tudor domains recognize and bind methylated histone H3 'Lys-4' residue. Double Tudor domain has an interdigitated structure and the unusual fold is required for its ability to bind methylated histone tails. Trimethylated H3 'Lys-4' is bound in a cage of 3 aromatic residues, 2 of which are from the Tudor domain 2, while the binding specificity is determined by side-chain interactions involving residues from the Tudor domain 1. The Tudor domains are able to bind trimethylated histone H3 'Lys-4', trimethylated histone H3 'Lys-9', di- and trimethylated H4 'Lys-20'.
Cellular localization
Nucleus.
Target information above from: UniProt accessionO75164
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- JmjC domain containing histone demethylation protein 3A antibody
- JmjC domain-containing histone demethylation protein 3A antibody
- JMJD2 antibody
see all
Database links
- Entrez Gene: 9682 Human
- Entrez Gene: 230674 Mouse
- Omim: 609764 Human
- SwissProt: O75164 Human
- SwissProt: Q8BW72 Mouse
- SwissProt: Q8K137 Mouse
- Unigene: 155983 Human
- Unigene: 234234 Mouse
Anti-KDM4A / JHDM3A / JMJD2A antibody images:
Western blot - KDM4A / JHDM3A / JMJD2A antibody (ab70786)
All lanes : Anti-KDM4A / JHDM3A / JMJD2A antibody (ab70786) at 0.04 µg/ml
Lane 1 : Nuclear extract from 293T cells at 50 µg
Lane 2 : Nuclear extract from 293T cells at 15 µg
Lane 3 : Nuclear extract from 293T cells at 5 µg
Lane 4 : Whole cell lysate from HeLa cells at 50 µg
Lane 5 : Whole cell lysate from NIH3T3 cells at 50 µg
Predicted band size : 121 kDa
Observed band size : 130 kDa (why is the actual band size different from the predicted?)
Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.
Immunoprecipitation - KDM4A / JHDM3A / JMJD2A antibody (ab70786)
Detection of Human KDM4A / JHDM3A / JMJD2A by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab70786 at 3 µg/mg lysate (Lane 1). Lane 2 represents IgG IP control. Subsequent Western blot detection of KDM4A / JHDM3A / JMJD2A was performed using ab70786 at 1 µg/ml.
References for Anti-KDM4A / JHDM3A / JMJD2A antibody (ab70786)
This product has been referenced in:
- Koshibu Ket al. Protein phosphatase 1 regulates the histone code for long-term memory. J Neurosci 29:13079-89 (2009). WB; Mouse.Read more (PubMed: 19828821) »
See 1 publication for this product
Publishing research using ab70786? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"