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Products:Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors
Overview
Product name
Anti-KDM4A / JHDM3A / JMJD2A antibody
See all KDM4A / JHDM3A / JMJD2A products (8) ...
Description
Rabbit polyclonal to KDM4A / JHDM3A / JMJD2A
Tested applications
IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide corresponding to a region between residues 350 and 400 of Human KDM4A / JHDM3A / JMJD2A (NP_055478.1).
Positive control
Human ovarian tumor tissue.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Methylation >> Lysine demethylation
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Jumonji containing proteins
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - KDM4A / JHDM3A / JMJD2A antibody (ab84518)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab84518 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
IHC-P
Application notes
IHC-P: 1/100 - 1/500.
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Histone demethylase that specifically demethylates 'Lys-9' and 'Lys-36' residues of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27' nor H4 'Lys-20'. Demethylates trimethylated H3 'Lys-9' and H3 'Lys-36' residue, while it has no activity on mono- and dimethylated residues. Demethylation of Lys residue generates formaldehyde and succinate. Participates in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1, respectively.
Tissue specificity
Ubiquitous.
Sequence similarities
Belongs to the JHDM3 histone demethylase family.
Contains 1 JmjC domain.
Contains 1 JmjN domain.
Contains 2 PHD-type zinc fingers.
Contains 2 Tudor domains.
Domain
The 2 Tudor domains recognize and bind methylated histone H3 'Lys-4' residue. Double Tudor domain has an interdigitated structure and the unusual fold is required for its ability to bind methylated histone tails. Trimethylated H3 'Lys-4' is bound in a cage of 3 aromatic residues, 2 of which are from the Tudor domain 2, while the binding specificity is determined by side-chain interactions involving residues from the Tudor domain 1. The Tudor domains are able to bind trimethylated histone H3 'Lys-4', trimethylated histone H3 'Lys-9', di- and trimethylated H4 'Lys-20'.
Cellular localization
Nucleus.
Target information above from: UniProt accessionO75164
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- JmjC domain containing histone demethylation protein 3A antibody
- JmjC domain-containing histone demethylation protein 3A antibody
- JMJD2 antibody
see all
Database links
- Entrez Gene: 9682 Human
- Omim: 609764 Human
- SwissProt: O75164 Human
- Unigene: 155983 Human
Anti-KDM4A / JHDM3A / JMJD2A antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - KDM4A / JHDM3A / JMJD2A antibody (ab84518)
ab84518, at 1/250 dilution, staining KDM4A / JHDM3A / JMJD2A in formalin-fixed, paraffin-embedded human ovarian tumor by Immunohistochemistry using DAB staining.
References for Anti-KDM4A / JHDM3A / JMJD2A antibody (ab84518)
ab84518 has not yet been referenced specifically in any publications.
Publishing research using ab84518? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"