Anti-KDM4B / JMJD2B antibody (ab27531)

Overview

• Product nameAnti-KDM4B / JMJD2B antibodySee all KDM4B / JMJD2B primary antibodies ...
• Description
  Rabbit polyclonal to KDM4B / JMJD2B
• Tested applicationsWB, ICC/IF more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse
• Immunogen

  Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human JMJD2B.

  Read Abcam's proprietary immunogen policy

  (Peptide available as ab30598.)

• Positive controlThis antibody gave a positive signal in the following lysates: Jurkat Whole Cell; Jurkat Nuclear; K562 Whole Cell and K562 Nuclear. This antibody gave a positive signal in IF/ICC in the following methanol fixed cell line: T47D.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 1% BSA, PBS, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Epigenetics and Nuclear Signaling
  • Nuclear Signaling Pathways
  • Nuclear Receptors
  • Co-activators/co-repressors

  • Epigenetics and Nuclear Signaling
  • Chromatin Modifying Enzymes
  • Jumonji containing proteins

Applications

Target

• RelevanceKDM4B / JMJD2B is a jumonji domain containing histone demethylase that specifically demethylates histone H3 at K9 and K36.
• Cellular localizationNuclear
• Database links
  • Entrez Gene: 23030 Human
  • Entrez Gene: 193796 Mouse
  • Omim: 609765 Human
  • SwissProt: O94953 Human
  • SwissProt: Q91VY5 Mouse

• Alternative names
  JHDM3B antibodyJmjC domain-containing histone demethylation protein 3B antibodyJMJD2B antibody

  Jumonji domain containing protein 2B antibodyJumonji domain-containing protein 2B antibodyKIAA0876 antibodyLysine-specific demethylase 4B antibody

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Anti-KDM4B / JMJD2B antibody images

• 

  Western blot - KDM4B / JMJD2B antibody (ab27531)
  All lanes : Anti-KDM4B / JMJD2B antibody (ab27531) at 1 µg/ml
  
  Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
  Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Nuclear Lysate (ab14844)
  Lane 3 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
  Lane 4 : K562 (Human erythromyeloblastoid leukemia cell line) Nuclear Lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
  
  Performed under reducing conditions.
  
  Predicted band size : 121 kDa
  Observed band size : 150 kDa (why is the actual band size different from the predicted?)
  Additional bands at : 105 kDa,55 kDa,80 kDa. We are unsure as to the identity of these extra bands.
  
  Exposure time : 3 minutes
• 

  Immunocytochemistry/ Immunofluorescence - Anti-KDM4B / JMJD2B antibody (ab27531)

  ICC/IF image of ab27531 stained T47D cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab27531 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.