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Products:Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors
Overview
Product name
Anti-KDM4B / JMJD2B antibody
See all KDM4B / JMJD2B products (5) ...
Description
Rabbit polyclonal to KDM4B / JMJD2B
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human JMJD2B.
(Peptide available as ab30598.)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
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Western blot - KDM4B / JMJD2B antibody (ab27531)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab27531 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 150 kDa (predicted molecular weight: 121 kDa).
Target
Relevance
KDM4B / JMJD2B is a jumonji domain containing histone demethylase that specifically demethylates histone H3 at K9 and K36.
Cellular localization
Nuclear
Alternative names
- JHDM3B antibody
- JmjC domain-containing histone demethylation protein 3B antibody
- JMJD2B antibody
see all
Database links
- Entrez Gene: 23030 Human
- Entrez Gene: 193796 Mouse
- Omim: 609765 Human
- SwissProt: O94953 Human
- SwissProt: Q91VY5 Mouse
Anti-KDM4B / JMJD2B antibody images:
Western blot - KDM4B / JMJD2B antibody (ab27531)
All lanes : Anti-KDM4B / JMJD2B antibody (ab27531) at 1 µg/ml
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Nuclear Lysate (ab14844)
Lane 3 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
Lane 4 : K562 (Human erythromyeloblastoid leukemia cell line) Nuclear Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 121 kDa
Observed band size : 150 kDa (why is the actual band size different from the predicted?)
Additional bands at : 105 kDa,55 kDa,80 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 3 minutes
The 150 kDa band observed is comparable to molecular weights seen with other commercially available antibodies to KDM4B/JMJD2B.
References for Anti-KDM4B / JMJD2B antibody (ab27531)
This product has been referenced in:
- Beyer Set al. The histone demethylases JMJD1A and JMJD2B are transcriptional targets of hypoxia-inducible factor HIF. J Biol Chem 283:36542-52 (2008). WB; Human.Read more (PubMed: 18984585) »
See 1 publication for this product
Publishing research using ab27531? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"