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Western blot - KDM4B / JMJD2B antibody (ab27531)
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Product Name
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KDM4B / JMJD2B antibody
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See all KDM4B / JMJD2B antibodies (2)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to KDM4B / JMJD2B
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human JMJD2B. (Peptide available as ab30598.)
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Reacts with
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Hu
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Predicted to react
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Ms
(Due to sequence homology)
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Tested applications
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WB
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Abreviews
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Application notes
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Recommended dilutions
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 121 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Jumonji containing proteins
Chromatin and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors
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Relevance
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KDM4B / JMJD2B is a jumonji domain containing histone demethylase that specifically demethylates histone H3 at K9 and K36.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this KDM4B / JMJD2B antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for KDM4B / JMJD2B antibody images, references, products related to ab27531 and other tools.
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KDM4B / JMJD2B antibody images:
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 Western blot - KDM4B / JMJD2B antibody (ab27531)
All lanes : KDM4B / JMJD2B antibody (ab27531) at 1 µg/ml
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Nuclear Lysate (ab14844)
Lane 3 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
Lane 4 : K562 (Human erythromyeloblastoid leukemia cell line) Nuclear Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 121 kDa
Observed band size : 150 kDa (why is the actual band size different from the predicted?)
Additional bands at : 105 kDa,55 kDa,80 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 3 minutes
The 150 kDa band observed is comparable to molecular weights seen with other commercially available antibodies to KDM4B/JMJD2B.
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References for KDM4B / JMJD2B antibody (ab27531)
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This product has been used in:
Beyer S et al. The histone demethylases JMJD1A and JMJD2B are transcriptional targets of hypoxia-inducible factor HIF. J Biol Chem 283:36542-52 (2008). WB; Human. PubMed: 18984585
If you publish research using ab27531 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to KDM4B / JMJD2B
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KDM4B / JMJD2B antibody - more information
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KDM4B / JMJD2B antibody - related products:
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KDM4B / JMJD2B antibody - other tools:
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