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Western blot - KDM4C / GASC1 / JMJD2C antibody (ab27532)
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Product Name
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KDM4C / GASC1 / JMJD2C antibody
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See all KDM4C / GASC1 / JMJD2C antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to KDM4C / GASC1 / JMJD2C
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 450 - 550 of Human GASC1 / JMJD2C. (Peptide available as ab30652.)
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Predicted to react
(species key)
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Hu
(Due to sequence homology)
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 120 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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This antibody gave a positive signal in the following Whole Cell Lysates:
HeLa (Human epithelial carcinoma cell line)
Jurkat (Human T cell lymphoblast-like cell line)
A431 (Human epithelial carcinoma cell line)
HEK293 (Human embryonic kidney cell line)
HepG2 (Human hepatocellular liver carcinoma cell line)
MCF7 (Human breast adenocarcinoma cell line)
SHSY-5Y (Human neuroblastoma cell line)
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Cellular localization
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Nuclear
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Research areas
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Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Other
Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Jumonji containing proteins
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Relevance
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KDM4C / JMJD2C, a jumonji domain containing protein, is a histone demethylase. JMJD2C specifically demethylates Lysine 9 and Lysine 36 residues of Histone H3, thereby playing a central role in regulating histone modifications.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Batch dependent within range: 0.40 - 0.60 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this KDM4C / GASC1 / JMJD2C antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for KDM4C / GASC1 / JMJD2C antibody images, references, products related to ab27532 and other tools.
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KDM4C / GASC1 / JMJD2C antibody images:
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 Western blot - KDM4C / GASC1 / JMJD2C antibody (ab27532)
All lanes : KDM4C / GASC1 / JMJD2C antibody (ab27532) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 120 kDa
Observed band size : 120 kDa
Additional bands at : 72 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 10 minutes
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Search PubMed (MEDLINE) for references to KDM4C / GASC1 / JMJD2C
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KDM4C / GASC1 / JMJD2C antibody - more information
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Customer reviews (feedback) regarding KDM4C / GASC1 / JMJD2C antibody |
Customer FAQs regarding KDM4C / GASC1 / JMJD2C antibody |
Protocols for KDM4C / GASC1 / JMJD2C antibody |
Price and availability of products related to KDM4C / GASC1 / JMJD2C antibody |
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KDM4C / GASC1 / JMJD2C antibody - related products:
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KDM4C / GASC1 / JMJD2C antibody - other tools:
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Contact Abcam with a Technical Enquiry about KDM4C / GASC1 / JMJD2C antibody |
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