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Anti-KDM4C / GASC1 / JMJD2C antibody (ab27532)

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Overview

Product name

Anti-KDM4C / GASC1 / JMJD2C antibody
See all KDM4C / GASC1 / JMJD2C products (5) ...

Description

Rabbit polyclonal to KDM4C / GASC1 / JMJD2C

Tested applications

WBmore details

Cross reactivity

Reacts with

Human

Immunogen

Synthetic peptide conjugated to KLH derived from within residues 450 - 550 of Human GASC1 / JMJD2C.

Read Abcam's proprietary immunogen policy

(Peptide available as ab30652.)

Positive control

This antibody gave a positive signal in the following Whole Cell Lysates: HeLa (Human epithelial carcinoma cell line) Jurkat (Human T cell lymphoblast-like cell line) A431 (Human epithelial carcinoma cell line) HEK293 (Human embryonic kidney cell line) HepG2 (Human hepatocellular liver carcinoma cell line) MCF7 (Human breast adenocarcinoma cell line) SHSY-5Y (Human neuroblastoma cell line)

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

Research areas

Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Other
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Jumonji containing proteins
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Methylation

  • Western blot - KDM4C / GASC1 / JMJD2C antibody (ab27532)Western blot - KDM4C / GASC1 / JMJD2C antibody (ab27532) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab27532 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Histone demethylase that specifically demethylates 'Lys-9' and 'Lys-36' residues of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27' nor H4 'Lys-20'. Demethylates trimethylated H3 'Lys-9' and H3 'Lys-36' residue, while it has no activity on mono- and dimethylated residues. Demethylation of Lys residue generates formaldehyde and succinate.

Tissue specificity

Overexpressed in several esophageal squamous cell carcinomas (ESCs).

Sequence similarities

Belongs to the JHDM3 histone demethylase family.
Contains 1 JmjC domain.
Contains 1 JmjN domain.
Contains 2 PHD-type zinc fingers.
Contains 2 Tudor domains.

Domain

The 2 Tudor domains recognize and bind methylated histones. Double Tudor domain has an interdigitated structure and the unusual fold is required for its ability to bind methylated histone tails.

Cellular localization

Nucleus.

Target information above from: UniProt accessionQ9H3R0 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • JmjC domain-containing histone demethylation protein 3C antibody
  • Jumonji domain-containing protein 2C antibody
  • bA146B14.1 antibody
  • GASC 1 protein antibody
  • GASC-1 protein antibody
  • GASC1 antibody
  • Gene amplified in squamous cell carcinoma 1 protein antibody
  • JHDM3C antibody
  • JmjC domain containing histone demethylation protein 3C antibody
  • JmjC domain-containing histone demethylation protein 3C antibody
  • JMJD2C antibody
  • Jumonji domain containing 2C antibody
  • Jumonji domain containing protein 2C antibody
  • Jumonji domain-containing protein 2C antibody
  • Kdm4c antibody
  • KDM4C_HUMAN antibody
  • Lysine (K) specific demethylase 4C antibody
  • Lysine-specific demethylase 4C antibody
see all

Database links

Anti-KDM4C / GASC1 / JMJD2C antibody images:

  Western blot - KDM4C / GASC1 / JMJD2C antibody (ab27532)

Western blot - KDM4C / GASC1 / JMJD2C antibody (ab27532)

All lanes : Anti-KDM4C / GASC1 / JMJD2C antibody (ab27532) at 1 µg/ml

Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate

Lysates/proteins at 10 µg per lane.

Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Performed under reducing conditions.

Predicted band size : 120 kDa
Observed band size : 120 kDa
Additional bands at : 72 kDa. We are unsure as to the identity of these extra bands.

Exposure time : 10 minutes

References for Anti-KDM4C / GASC1 / JMJD2C antibody (ab27532)

ab27532 has not yet been referenced specifically in any publications.

Publishing research using ab27532? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"