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Overview
Product name
Anti-KDM4C / GASC1 / JMJD2C antibody
See all KDM4C / GASC1 / JMJD2C products (5) ...
Description
Rabbit polyclonal to KDM4C / GASC1 / JMJD2C
Tested applications
IHC-P, WBmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide corresponding to a region within the internal sequence amino acids 755-804 (CLCNLRGGAL KQTKNNKWAH VMCAVAVPEV RFTNVPERTQ IDVGRIPLQR) of Human KDM4C / GASC1 / JMJD2C, NP_055876.
CLCNLRGGAL KQTKNNKWAH VMCAVAVPEV RFTNVPERTQ IDVGRIPLQR
Positive control
HepG2 cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
-
Western blot - KDM4C / GASC1 / JMJD2C antibody (ab85454)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab85454 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at an assay dependent concentration.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 100 kDa (predicted molecular weight: 120 kDa).
Target
Function
Histone demethylase that specifically demethylates 'Lys-9' and 'Lys-36' residues of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27' nor H4 'Lys-20'. Demethylates trimethylated H3 'Lys-9' and H3 'Lys-36' residue, while it has no activity on mono- and dimethylated residues. Demethylation of Lys residue generates formaldehyde and succinate.
Tissue specificity
Overexpressed in several esophageal squamous cell carcinomas (ESCs).
Sequence similarities
Belongs to the JHDM3 histone demethylase family.
Contains 1 JmjC domain.
Contains 1 JmjN domain.
Contains 2 PHD-type zinc fingers.
Contains 2 Tudor domains.
Domain
The 2 Tudor domains recognize and bind methylated histones. Double Tudor domain has an interdigitated structure and the unusual fold is required for its ability to bind methylated histone tails.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ9H3R0
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- bA146B14.1 antibody
- GASC 1 protein antibody
- GASC-1 protein antibody
see all
Database links
- Entrez Gene: 23081 Human
- Entrez Gene: 76804 Mouse
- Omim: 605469 Human
- SwissProt: Q9H3R0 Human
- SwissProt: Q8VCD7 Mouse
- Unigene: 709425 Human
- Unigene: 209059 Mouse
Anti-KDM4C / GASC1 / JMJD2C antibody images:
Western blot - KDM4C / GASC1 / JMJD2C antibody (ab85454)
Anti-KDM4C / GASC1 / JMJD2C antibody (ab85454) at 1 µg/ml + HepG2 cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 120 kDa
Observed band size : 100 kDa (why is the actual band size different from the predicted?)
Gel concentration 6-18%
References for Anti-KDM4C / GASC1 / JMJD2C antibody (ab85454)
ab85454 has not yet been referenced specifically in any publications.
Publishing research using ab85454? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"