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Western blot - KDM4D / JMJD2D antibody (ab63199)
Western blot - KDM4D / JMJD2D antibody (ab63199)
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Product Name 

KDM4D / JMJD2D antibody

 

See all KDM4D / JMJD2D antibodies (2)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to KDM4D / JMJD2D

Immunogen 

Synthetic peptide from the C-terminal region of human KDM4D / JMJD2D, conjugated to KLH.

Reacts with 
(species key)

Hu

Tested applications 
(see key)

ELISA, WB


Abreviews 

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(see key)


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Application notes 
(see key)

Recommended dilutions
ELISA: 1/1000.
WB: 1/250 - 1/500. Detects a band of approximately 52 kDa (predicted molecular weight: 58 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Hela cell line lysate.

Cellular localization 

Nuclear

Research areas 

Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Methylation >> Lysine demethylation
Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Jumonji containing proteins

Relevance 

KDM4D / JMJD2D is a histone demethylase that contains a jumonji domain. KDM4D specifically demethylates mono and di methyl histone H3 at K9.

 

Alternative names KDM4D / JMJD2D antibody (ab63199)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

55693    (Human)

Omim

    

609766    (Human)

SwissProt

    

Q6B0I6    (Human)

Unigene

    

503598    (Human)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Protein A purified

Storage buffer 

Preservative: 0.09% Sodium Azide
Constituents: PBS

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

ab63199 was purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Form 

Liquid

Concentration 

0.250 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this KDM4D / JMJD2D antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for KDM4D / JMJD2D antibody images, references, products related to ab63199 and other tools.

 

KDM4D / JMJD2D antibody images:

Product image  Western blot - KDM4D / JMJD2D antibody (ab63199)

Western blot - KDM4D / JMJD2D antibody (ab63199)

KDM4D / JMJD2D antibody (ab63199) at 1/250 dilution + Hela cell line lysate.

Predicted band size : 58 kDa
Observed band size : 52 kDa (why is the actual band size different from the predicted?)

 
 

Search PubMed (MEDLINE) for references to KDM4D / JMJD2D

 

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KDM4D / JMJD2D antibody - related products:

Products for Western blot

Loading controls

Lysates

 

Positive Controls for KDM4D / JMJD2D antibody

ab14655

  

HeLa (Human epithelial carcinoma cell line) Nuclear Lysate

 

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Other KDM4D / JMJD2D Antibodies

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KDM4D / JMJD2D antibody - other tools:

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