KDM5A / Jarid1A / RBBP2 antibody - ChIP Grade (ab45301)

Product Name 

KDM5A / Jarid1A / RBBP2 antibody - ChIP Grade

Product type 

Primary antibodies

Description 

Rabbit polyclonal to KDM5A / Jarid1A / RBBP2 - ChIP Grade

Immunogen 

Protein fragment spanning 243aa of RBP2-H1

Reacts with 
(species key)

Hu

Specificity 

This antibody detects Retinoblastoma binding protein 2 homolog 1 only.

Tested applications 
(see key)

ChIP, IHC-P, IP

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ChIP: Use at an assay dependent dilution (PMID 18940868).
IHC-P: 1/100 (incubate at 100 ^oC for 10 min with citrate buffer pH6.0 before incubating with primary antibody).
IP: Use at an assay dependent dilution.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Nuclear

Research areas 

Chromatin and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Rb family
Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Jumonji containing proteins
Chromatin and Nuclear Signaling >> Transcription >> Co-factors
Chromatin and Nuclear Signaling >> Transcription >> Transcription Factors
Chromatin and Nuclear Signaling >> Transcription >> Other factors
Chromatin and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors
Chromatin and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol II Transcription >> Other

Relevance 

KDM5A / JARID1A / RBBP2 is a ubiquitously expressed nuclear protein. KDM5A specifically demethylates di and tri methyl histone H3 at K4.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Protein A purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.500 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

References for KDM5A / Jarid1A / RBBP2 antibody - ChIP Grade (ab45301)

  This product has been used in: (two most recent references)

Gatta R & Mantovani R NF-Y substitutes H2A-H2B on active cell-cycle promoters: recruitment of CoREST-KDM1 and fine-tuning of H3 methylations. Nucleic Acids Res 36:6592-607 (2008). ChIP; Human. PubMed: 18940868

Roesch A et al. Retinoblastoma-binding protein 2-homolog 1: a retinoblastoma-binding protein downregulated in malignant melanomas. Mod Pathol 18:1249-57 (2005). PubMed: 15803180
If you publish research using ab45301 please let us know so that we can cite the reference on this datasheet.

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