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Anti-KDM5A / Jarid1A / RBBP2 antibody - ChIP Grade (ab65769)

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Overview

Product name

Anti-KDM5A / Jarid1A / RBBP2 antibody - ChIP Grade
See all KDM5A / Jarid1A / RBBP2 products (6) ...

Description

Rabbit polyclonal to KDM5A / Jarid1A / RBBP2 - ChIP Grade

Tested applications

WB, IP, ChIPmore details

Cross reactivity

Reacts with

Mouse, Human

Immunogen

Synthetic peptide, corresponding to amino acids 1440-1687 of Human KDM5A / Jarid1A / RBBP2

Positive control

HeLa nuclear extract

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: Whole serum

Purity

Whole antiserum

Clonality

Polyclonal

Isotype

IgG

Research areas

Epigenetics and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Jumonji containing proteins
Epigenetics and Nuclear Signaling >> Transcription >> Co-factors
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors

  • Western blot - KDM5A / Jarid1A / RBBP2 antibody (ab65769)Western blot - KDM5A / Jarid1A / RBBP2 antibody (ab65769) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab65769 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/1000Detects a band...Read more →

    WB: 1/1000Detects a band of approximately 190 kDa (predicted molecular weight: 196 kDa).

  • ShowHide

    IP

     IP: Use at an assay depe...Read more →

    IP: Use at an assay dependent dilution.

  • ShowHide

    ChIP

     ChIP: Use at an assay de...Read more →

    ChIP: Use at an assay dependent dilution. (PubMed: 20851996)

Target

Function

Histone demethylase that specifically demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-4'. May stimulate transcription mediated by nuclear receptors. May be involved in transcriptional regulation of Hox proteins during cell differentiation. May participate in transcriptional repression of cytokines such as CXCL12.

Sequence similarities

Belongs to the JARID1 histone demethylase family.
Contains 1 ARID domain.
Contains 1 JmjC domain.
Contains 1 JmjN domain.
Contains 3 PHD-type zinc fingers.

Domain

The GSGFP motif is required for the interaction with SUZ12.

Cellular localization

Nucleus > nucleolus. Occupies promoters of genes involved in RNA metabolism and mitochondrial function.

Target information above from: UniProt accessionP29375 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Histone demethylase JARID1A antibody
  • JARID1A antibody
  • Jumonji/ARID domain containing protein 1A antibody
  • Jumonji/ARID domain-containing protein 1A antibody
  • Kdm5a antibody
  • KDM5A_HUMAN antibody
  • Lysine-specific demethylase 5A antibody
  • RBBP-2 antibody
  • RBP2 antibody
  • Retinoblastoma binding protein 2 antibody
  • Retinoblastoma-binding protein 2 antibody
see all

Database links

Anti-KDM5A / Jarid1A / RBBP2 antibody - ChIP Grade images:

  Western blot - KDM5A / Jarid1A / RBBP2 antibody (ab65769)

Western blot - KDM5A / Jarid1A / RBBP2 antibody (ab65769)

Anti-KDM5A / Jarid1A / RBBP2 antibody - ChIP Grade (ab65769) at 1/1000 dilution + Nuclear extract of HeLa cells

Predicted band size : 196 kDa
Observed band size : 190 kDa (why is the actual band size different from the predicted?)

References for Anti-KDM5A / Jarid1A / RBBP2 antibody - ChIP Grade (ab65769)

This product has been referenced in:

  • Liefke Ret al. Histone demethylase KDM5A is an integral part of the core Notch-RBP-J repressor complex. Genes Dev 24:590-601 (2010). ChIP; Mouse.Read more (PubMed: 20231316) »
  • Flowers Set al. Transcriptional activation by pRB and its coordination with SWI/SNF recruitment. Cancer Res 70:8282-7 (2010). ChIP; Mouse.Read more (PubMed: 20851996) »

See all 2 publications for this product

Publishing research using ab65769? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"