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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab70892? Please let us know so that we can cite the reference in this datasheet
ab70892 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - KDM5A / Jarid1A / RBBP2 antibody (ab70892)
All lanes : Anti-KDM5A / Jarid1A / RBBP2 antibody (ab70892) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
developed using the ECL technique
Predicted band size : 196 kDa
Observed band size : 196 kDa
Exposure time : 3 minutes
Immunoprecipitation - KDM5A / Jarid1A / RBBP2 antibody (ab70892)
HeLa whole cell lysate (at 1 mg for IP, 20% of IP loaded), with ab70892 at 1µg/ml for WB, after IP at 3 µg/mg lysate.
Detection: Chemiluminescence with an exposure time of 10 seconds.
Immunocytochemistry/ Immunofluorescence-Anti-KDM5A / Jarid1A / RBBP2 antibody(ab70892)
ICC/IF image of ab70892 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70892, 5µg/ml) overnight at +4ºC. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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