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antibody not working in ChIP with positive control mouse cell lines; ChIP control with anti-histone h3 antibody works fine with same samples; |
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ANSWER: |
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab70892. |
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Dear abcam technical support team: Our customer advances an enquiry about ab70892 (Anti-KDM5A / Jarid1A / RBBP2 antibody). She would like to know the currently in stock lot number of this antibody and its corresponding quantity. Would you please help this customer to solve the problem? Thanks for your kindly help Best regards, |
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ANSWER: |
Thank you for contacting us and your interest in our products. Currently we have two lots of Anti-KDM5A / Jarid1A / RBBP2 antibody (ab70892) in stock. These are both of lot number GR70517-1. I hope this information has been of help. If your customerneeds any further information please do not hesitate to let me know. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
All lanes : Anti-KDM5A / Jarid1A / RBBP2 antibody (ab70892) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
developed using the ECL technique
Predicted band size : 196 kDa
Observed band size : 196 kDa
Exposure time : 3 minutes
HeLa whole cell lysate (at 1 mg for IP, 20% of IP loaded), with ab70892 at 1µg/ml for WB, after IP at 3 µg/mg lysate.
Detection: Chemiluminescence with an exposure time of 10 seconds.
ICC/IF image of ab70892 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70892, 5µg/ml) overnight at +4ºC. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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