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Western blot - KDM5A / Jarid1A / RBBP2 antibody (ab70892)
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Immunoprecipitation - KDM5A / Jarid1A / RBBP2 antibody (ab70892)
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Product Name
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KDM5A / Jarid1A / RBBP2 antibody
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See all KDM5A / Jarid1A / RBBP2 antibodies (6)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to KDM5A / Jarid1A / RBBP2
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Immunogen
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A region between residue 1675 and the C-terminus (residue 1722) of human KDM5A / Jarid1A / RBBP2. (NP_005047.1)
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Reacts with
(species key)
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Hu, Ms
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Tested applications
(see key)
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IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IP: Use at a concentration of 2 - 5 µg/ml.
WB: 1/2000 - 1/10000. Detects a band of approximately 196 kDa (predicted molecular weight: 196 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HeLa, 293T and NIH3T3 whole cell lysates.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Jumonji containing proteins
Chromatin and Nuclear Signaling >> Transcription >> Co-factors
Chromatin and Nuclear Signaling >> Transcription >> Other factors
Chromatin and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors
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Relevance
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KDM5A / JARID1A / RBBP2 is a ubiquitously expressed nuclear protein. KDM5A specifically demethylates di and tri methyl histone H3 at K4.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.200 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this KDM5A / Jarid1A / RBBP2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for KDM5A / Jarid1A / RBBP2 antibody images, references, products related to ab70892 and other tools.
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KDM5A / Jarid1A / RBBP2 antibody images:
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 Western blot - KDM5A / Jarid1A / RBBP2 antibody (ab70892)
All lanes : KDM5A / Jarid1A / RBBP2 antibody (ab70892) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
developed using the ECL technique
Predicted band size : 196 kDa
Observed band size : 196 kDa
Exposure time : 3 minutes
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Immunoprecipitation - KDM5A / Jarid1A / RBBP2 antibody (ab70892)
HeLa whole cell lysate (at 1 mg for IP, 20% of IP loaded), with ab70892 at 1µg/ml for WB, after IP at 3 µg/mg lysate.
Detection: Chemiluminescence with an exposure time of 10 seconds.
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Search PubMed (MEDLINE) for references to KDM5A / Jarid1A / RBBP2
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KDM5A / Jarid1A / RBBP2 antibody - more information
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Customer reviews (feedback) regarding KDM5A / Jarid1A / RBBP2 antibody |
Customer FAQs regarding KDM5A / Jarid1A / RBBP2 antibody |
Protocols for KDM5A / Jarid1A / RBBP2 antibody |
Price and availability of products related to KDM5A / Jarid1A / RBBP2 antibody |
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KDM5A / Jarid1A / RBBP2 antibody - related products:
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KDM5A / Jarid1A / RBBP2 antibody - other tools:
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