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Products:Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors
Overview
Product name
Anti-KDM5A / Jarid1A / RBBP2 antibody
See all KDM5A / Jarid1A / RBBP2 products (6) ...
Description
Rabbit polyclonal to KDM5A / Jarid1A / RBBP2
Tested applications
WB, IP, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
A region between residue 1675 and the C-terminus (residue 1722) of human KDM5A / Jarid1A / RBBP2. (NP_005047.1)
Positive control
HeLa, 293T and NIH3T3 whole cell lysates.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Jumonji containing proteins
Epigenetics and Nuclear Signaling >> Transcription >> Co-factors
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors
Applications
Show applications key
Our Abpromise guarantee covers the use of ab70892 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000 - 1/10000.Detects a band of approximately 196 kDa (predicted molecular weight: 196 kDa).
IP: Use a concentration of 2 - 5 µg/ml.
ICC/IF: Use a concentration of 5 µg/ml
Target
Function
Histone demethylase that specifically demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-4'. May stimulate transcription mediated by nuclear receptors. May be involved in transcriptional regulation of Hox proteins during cell differentiation. May participate in transcriptional repression of cytokines such as CXCL12.
Sequence similarities
Belongs to the JARID1 histone demethylase family.
Contains 1 ARID domain.
Contains 1 JmjC domain.
Contains 1 JmjN domain.
Contains 3 PHD-type zinc fingers.
Domain
The GSGFP motif is required for the interaction with SUZ12.
Cellular localization
Nucleus > nucleolus. Occupies promoters of genes involved in RNA metabolism and mitochondrial function.
Target information above from: UniProt accessionP29375
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Histone demethylase JARID1A antibody
- JARID1A antibody
- Jumonji/ARID domain containing protein 1A antibody
see all
Database links
- Entrez Gene: 5927 Human
- Entrez Gene: 214899 Mouse
- Omim: 180202 Human
- SwissProt: P29375 Human
- SwissProt: Q3UXZ9 Mouse
- Unigene: 76272 Human
- Unigene: 404761 Mouse
- Unigene: 463658 Mouse
Anti-KDM5A / Jarid1A / RBBP2 antibody images:
Western blot - KDM5A / Jarid1A / RBBP2 antibody (ab70892)
All lanes : Anti-KDM5A / Jarid1A / RBBP2 antibody (ab70892) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
developed using the ECL technique
Predicted band size : 196 kDa
Observed band size : 196 kDa
Exposure time : 3 minutes
Immunoprecipitation - KDM5A / Jarid1A / RBBP2 antibody (ab70892)
HeLa whole cell lysate (at 1 mg for IP, 20% of IP loaded), with ab70892 at 1µg/ml for WB, after IP at 3 µg/mg lysate.
Detection: Chemiluminescence with an exposure time of 10 seconds.
Immunocytochemistry/ Immunofluorescence-Anti-KDM5A / Jarid1A / RBBP2 antibody(ab70892)
ICC/IF image of ab70892 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70892, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-KDM5A / Jarid1A / RBBP2 antibody (ab70892)
ab70892 has not yet been referenced specifically in any publications.
Publishing research using ab70892? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"