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ChIP - KDM5B / PLU1 / Jarid1B antibody - ChIP Grade (ab50958)
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Immunocytochemistry/ Immunofluorescence - KDM5B / PLU1 / Jarid1B antibody - ChIP Grade (ab50958)
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Product Name
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KDM5B / PLU1 / Jarid1B antibody - ChIP Grade
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See all KDM5B / PLU1 / Jarid1B antibodies (5)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to KDM5B / PLU1 / Jarid1B - ChIP Grade
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Immunogen
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Recombinant fragment, corresponding to amino acids 125-355 of Human PLU1 / Jarid1B
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Reacts with
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Hu, Ms
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Tested applications
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ChIP, ICC/IF, IP, WB
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Abreviews
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Application notes
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Recommended dilutions
ChIP: Use at an assay dependent dilution.
ICC/IF: 1/1000.
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 190 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Jumonji containing proteins
Chromatin and Nuclear Signaling >> Transcription >> Co-factors
Chromatin and Nuclear Signaling >> Transcription >> Other factors
Chromatin and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors
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Relevance
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KDM5B / PLU1 / Jarid1b is a novel breast cancer associated nuclear protein containing highly conserved domains, including JmjC and JmjN motifs. KDM5B interacts with Brain Factor 1 (BF1) and Pax9. KDM5B specifically demethylates mono, di and tri methyl histone H3 at K4.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Whole antiserum
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this KDM5B / PLU1 / Jarid1B antibody - ChIP Grade is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for KDM5B / PLU1 / Jarid1B antibody - ChIP Grade images, references, products related to ab50958 and other tools.
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KDM5B / PLU1 / Jarid1B antibody - ChIP Grade images:
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 ChIP - KDM5B / PLU1 / Jarid1B antibody - ChIP Grade (ab50958)
Chromatin was prepared from a whole tissue lysate of mouse skin epidermis according to the Abcam X-ChIP protocol. Cells were fixed with 1% formaldehyde for 10 min. An anti-intergrin V antibody was added to the control, which showed no application. Ab59058 was diluted 1/800 and incubated with the sample for 12 hours at 4°C. The immunoprecipitated DNA was quantified by semiquantitative PCR. This image is courtesy of an anonymous Abreview
See Abreview
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Immunocytochemistry/ Immunofluorescence - KDM5B / PLU1 / Jarid1B antibody - ChIP Grade (ab50958)
ICC/IF image of ab50958 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab50958, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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References for KDM5B / PLU1 / Jarid1B antibody - ChIP Grade (ab50958)
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This product has been used in: (two most recent references)
Aslan JE et al. Akt and 14-3-3 control a PACS-2 homeostatic switch that integrates membrane traffic with TRAIL-induced apoptosis. Mol Cell 34:497-509 (2009). ChIP; Human. PubMed: 19481529
Gatta R & Mantovani R NF-Y substitutes H2A-H2B on active cell-cycle promoters: recruitment of CoREST-KDM1 and fine-tuning of H3 methylations. Nucleic Acids Res 36:6592-607 (2008). ChIP; Human. PubMed: 18940868
See all 3 publication references for this product.
If you publish research using ab50958 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to KDM5B / PLU1 / Jarid1B
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KDM5B / PLU1 / Jarid1B antibody - ChIP Grade - more information
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KDM5B / PLU1 / Jarid1B antibody - ChIP Grade - related products:
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KDM5B / PLU1 / Jarid1B antibody - ChIP Grade - other tools:
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Contact Abcam with a Technical Enquiry about KDM5B / PLU1 / Jarid1B antibody - ChIP Grade |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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